Neuroblastoma, brain, derived from metastatic site: bone marrow. From 6 year old female donor
MEM Eagle (also called EMEM) was developed in 1959 for cultivation of HeLa and L cells. Amino acid concentrations conform closely to the protein composition of human cells. Higher concentrations of nutrients permit longer periods between feedings. EMEM has vitamin concentrations 2-5X greater than BME and higher amino acid concentrations than BME. EMEM is suitable for culturing a broad spectrum of mammalian cells.MEM Eagle is available with Hanks' or Earle's salts.MEM-Hanks (12-127F or 12-137F) does not a require CO2 enriched atmosphere. Joklik's modification is intended for suspension culture.Storage = 2ºC to 8ºC (or 15-30ºC for products 12-125, 12-136, 12-684, 12-668, 12-127 and 12-137)MEM Eagle is available in several variations: - with Earle's BSS12-611 - with L-glutamine12-125 - without L-glutamine12-622 - with non-essential amino acids (NEAA), and sodium pyruvate, without L-glutamine12-136 - with L-glutamine and HEPES12-736 - with L-glutamine, HEPES, NEAA, gentamycin, pen/strep, ampB and 2% FBS12-684 - 10x media without sodium bicarbonate or L-glutamine12-668 - 2x media without L-glutamine or phenol red (virus plaquing medium)06-174 - without calcium, with NEAA and L-glutamine- with Hank's BSS12-127 - without L-glutamine12-137 - with HEPES, without L-glutamine- Joklik's Formulation04-719
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