SK-N-AS
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Neuroblastoma, brain, derived from metastatic site: bone marrow. From 6 year old female donor
Cell Type:
Neuroblast
Tissue Origin:
brain
Species:
human
Research Area:
Cancer Research/Cell Biology
Basic Research
Cell Characteristics:
Adherent
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
Filter:
Any Substrate
Any Platform
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
SF
FF-104
2e5
91% ±1
89% ±4
Plasmid (general)
0.4 µg
20 µl
4D X-Unit
SF
FF-104
1e6
95% ±1
87% ±5
Plasmid (general)
2 µg
100 µl
4D X-Unit
SF
FF-104
2e5
90-92%
85-93%
Plasmid (general)
0.4 µg
20 µl
96-well
Citations
Neuronal Hypoxia Induces Hsp40-Mediated Nuclear Import of Type 3 Deiodinase As an Adaptive Mechanism to Reduce Cellular Metabolism
Categories:
Transfection
Authors:
Jo S, Kalló I, Bardóczi Z, Arrojo e Drigo R, Zeöld A, Liposits Z, Oliva A, Lemmon VP, Bixby JL, Gereben B, Bianco AC.
In:
J Neurosci (2012) 32(25): 8491-500