Adipocyte (pre), human diabetes Tp.2

Preadipocytes are precursor cells that develop into adipocytes when fully differentiated. Adipocytes perform essential functions of energy metabolism and are characterized by the accumulation of intracellular triglycerides.

Preadipocyte Cells are isolated from subcutaneous or visceral fat. Subcutaneous fat is often found attached to skin in the lower abdomen area. Visceral preadipocytes are isolated from adipose tissue associated with internal organs, such as the bladder or kidney.

Relative to subcutaneous fat, visceral fat deposits are mobilized at a higher rate to produce serum fatty acids which contribute to insulin resistance, Diabetes Type 2, and other related cardiovascular disorders.

Diabetes mellitus type 2 – formerly non-insulin-dependent diabetes mellitus (NIDDM) or adult-onset diabetes – is a metabolic disorder that is characterized by high blood glucose in the context of insulin resistance and relative insulin deficiency.

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Recommended Media

Our Preadipocyte Growth Media-2 (PGM™-2) is optimized for preadipocyte proliferation, and differentiation in only 10 days of culture time. The media contains no glitazones that can interfere with PPAR-gamma activity measurements. The media used with our cells compose a robust system that requires no media changes during the culture time to differentiate.

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
P1 CA-137 1e5 28-43% 28-56% Plasmid (general) 400 ng 20 µl 4D X-Unit
P1 CA-158 1e5 49-70% 45-75% Plasmid (general) 400 ng 20 µl 4D X-Unit