Endothelial, colony forming, human(ECFC)

Cryopreserved ECFC are clonally expanded cells isolated from human umbilical cord blood.

Cell Type:
Endo. Progenitor
Tissue Origin:
extra-embryonic
Species:
human
Research Area:
Stem Cells
Cell Characteristics:
Adherent

Recommended Media

Endothelial cell media are low serum media optimized for the proliferation of endothelial cells.

EGM-2 is a refined version of EGM.
 - Does not contain BBE
 - Final serum concentration is 2%
 - Improved cell proliferation over EGM

EGM-2 can be used to grow all of Clonetics Endothelial Cells except microvascular, coronary artery, umbilical artery, and iliac artery

Transfection Information

In case no data are shown for the selected cell type, please take a look at our optimization strategy or contact our Scientific Support Teams to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Citations

Categories:
Serum-free and Speciality Media 
Authors:
Lyssia Belarif, Bernard Vanhove, Nicolas Poirier 
In:
J Clin Invest (2019) 129: 5 
Categories:
Primary Cells and Media 
Authors:
Falcon BL, Swearingen M, Gough WH, Lee L, Foreman R, Uhlik M, Hanson JC, Lee JA, McClure DB, Chintharlapalli S 
In:
PLoS ONE (2014) 9(9): ePub 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Falcon BL, O'Clair B, McClure D, Evans GF, Stewart J, Swearingen ML, Chen Y, Allard K, Lee LN, Neote K, McEwen DP, Uhlik MT, Chintharlapalli S 
In:
J Hematol Oncol (2013) 6: 31 
Categories:
Primary Cells and Media 
Authors:
Willard MD, Lajiness ME, Wulur IH, Feng B, Swearingen ML, Uhlik MT, Kinzler KW, Velculescu VE, Sjöblom T, Markowitz SD, Powell SM, Vogelstein B, Barber TD. 
In:
Mol Cancer Res (2012) 10(6): 739-49 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Meier T, Uhlik M, Chintharlapalli S, Dowless M, Van Horn R, Stewart J, Blosser W, Cook J, Young D, Ye X, Evans G, Credille K, Ballard D, Huber L, Capen A, Chedid M, Ilaria R Jr, Smith MC, Stancato L. 
In:
Mol Cancer Ther (2011) 10(11): 2168-78