Bone marrow stroma, human

When bone marrow cells are put into culture under specific conditions, a stromal layer of adherent cells develops over a few weeks.

The stromal layer is composed of many cell types, including fibroblasts, mesenchymal stem cells, adipocytes, endothelial cells and macrophages. These stromal cells function as a feeder layer for hematopoietic progenitors, allowing proliferation and differentiation of progenitors to continue for weeks in these cultures with no addition of exogenous cytokines.

This long-term culture system allows researchers to study an in vitro model of the bone marrow microenvironment, including cell-cell interactions, adhesion molecules and cytokine secretion.These and many other factors allow for the tight regulation of blood cell production, progenitor cell commitment and differentiation, and stem cell renewal.

Cell Type:
Blood/Bone Marrow SC
Tissue Origin:
bone marrow
Species:
human
Research Area:
Immunotherapy / Hematology
Stem Cells
Cell Characteristics:
Adherent

Transfection Information

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Citations (8)

Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Sergei Paylian 
In:
BAOJ Cancer Research & Therapy (2015) 1(2): 1 
Categories:
 
Authors:
Hale SJ, Hale AB, Zhang Y, Sweeney D, Fisher N, van der Garde M, Grabowska R, Pepperell E, Channon K, Martin-Rendon E, Watt SM 
In:
Br J of Haematol (2015) 169(4): 552-64 
Categories:
Primary Cells and Media 
Authors:
Reikvam H, Nepstad I, Bruserud Ø, Hatfield KJ 
In:
OncoTarget (2013) 4(6): 830-43 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Liu Q, Xi Y, Terry T, So SP, Mohite A, Zhang J, Wu G, Liu X, Cheng J, Ruan KH, Willerson JT, Dixon RA. 
In:
J Cell Physiol (2012) 22(7): 2907-16 
Categories:
Primary Cells and Media 
Authors:
Nakamori Y, Liu B, Ohishi K, Suzuki K, Ino K, Matsumoto T, Masuya M, Nishikawa H, Shiku H, Hamada H, Katayama N 
In:
Br J of Haematol (2012) 157(6): 674-86 
Categories:
Primary Cells and Media 
Authors:
Wara AK, Croce K, Foo S, Sun X, Icli B, Tesmenitsky Y, Esen F, Rosenzweig A, Feinberg MW. 
In:
Blood (2011) 118(24): 6461-4 
Categories:
Primary Cells and Media 
Authors:
Kamura S, Matsumoto Y, Fukushi JI, Fujiwara T, Iida K, Okada Y, Iwamoto Y. 
In:
Br J Cancer (2010) 103(3): 370-81 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Jamieson WL, Shimizu S, D\'Ambrosio JA, Meucci O, Fatatis A 
In:
Cancer Res (2008) 68(6): 1715-22 
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