Bone marrow stroma, human

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When bone marrow cells are put into culture under specific conditions, a stromal layer of adherent cells develops over a few weeks.

The stromal layer is composed of many cell types, including fibroblasts, mesenchymal stem cells, adipocytes, endothelial cells and macrophages. These stromal cells function as a feeder layer for hematopoietic progenitors, allowing proliferation and differentiation of progenitors to continue for weeks in these cultures with no addition of exogenous cytokines.

This long-term culture system allows researchers to study an in vitro model of the bone marrow microenvironment, including cell-cell interactions, adhesion molecules and cytokine secretion.These and many other factors allow for the tight regulation of blood cell production, progenitor cell commitment and differentiation, and stem cell renewal.

Cell Type:
Blood/Bone Marrow SC
Tissue Origin:
bone marrow
Species:
human
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Research Area:
Immunotherapy / Hematology
Stem Cells
Cell Characteristics:
Adherent

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Citations (7)

CXCR2 modulates bone marrow vascular repair and haematopoietic recovery post-transplant
Categories:
 
Authors:
Hale SJ, Hale AB, Zhang Y, Sweeney D, Fisher N, van der Garde M, Grabowska R, Pepperell E, Channon K, Martin-Rendon E, Watt SM 
In:
Br J of Haematol (2015) 169(4): 552-64 
Pharmacological targeting of the PI3K/mTOR pathway alters the release of angioregulatory mediators both from primary human acute myeloid leukemia cells and their neighboring stromal cells
Categories:
Primary Cells and Media 
Authors:
Reikvam H, Nepstad I, Bruserud Ø, Hatfield KJ 
In:
OncoTarget (2013) 4(6): 830-43 
Engineered endothelial progenitor cells that overexpress prostacyclin protect vascular cells.
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Liu Q, Xi Y, Terry T, So SP, Mohite A, Zhang J, Wu G, Liu X, Cheng J, Ruan KH, Willerson JT, Dixon RA. 
In:
J Cell Physiol (2012) 22(7): 2907-16 
Human bone marrow stromal cells simultaneously support B and T/NK lineage development from human haematopoietic progenitors: a principal role for flt3 ligand in lymphopoiesis.
Categories:
Primary Cells and Media 
Authors:
Nakamori Y, Liu B, Ohishi K, Suzuki K, Ino K, Matsumoto T, Masuya M, Nishikawa H, Shiku H, Hamada H, Katayama N 
In:
Br J of Haematol (2012) 157(6): 674-86 
Bone marrow-derived CMPs and GMPs represent highly functional proangiogenic cells: implications for ischemic cardiovascular disease
Categories:
Primary Cells and Media 
Authors:
Wara AK, Croce K, Foo S, Sun X, Icli B, Tesmenitsky Y, Esen F, Rosenzweig A, Feinberg MW. 
In:
Blood (2011) 118(24): 6461-4 
Basic fibroblast growth factor in the bone microenvironment enhances cell motility and invasion of Ewing\\\'s sarcoma family of tumours by activating the FGFR1-PI3K-Rac1 pathway
Categories:
Primary Cells and Media 
Authors:
Kamura S, Matsumoto Y, Fukushi JI, Fujiwara T, Iida K, Okada Y, Iwamoto Y. 
In:
Br J Cancer (2010) 103(3): 370-81 
CX3CR1 is expressed by prostate epithelial cells and androgens regulate the levels of CX3CL1/fractalkine in the bone marrow: potential role in prostate cancer bone tropism.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Jamieson WL, Shimizu S, D\'Ambrosio JA, Meucci O, Fatatis A 
In:
Cancer Res (2008) 68(6): 1715-22 
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