Fibroblast, ventricular cardiac, human

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Cardiac fibroblasts isolated from ventricular cardiac tissue

Cell Type:
Fibroblast
Tissue Origin:
heart
Species:
human
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Research Area:
Cancer Research/Cell Biology
Cardiovascular
Cell Characteristics:
Adherent

Recommended Media

Fibroblast Growth Medium-3

FGM-3 is specially formulated for Cardiac Fibroblast growth.
Contains a vial of FBS for a final serum concentration of 10%

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
P2 EN-150 1e5 100% 97% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
P2 DS-150 1e5 96% 99% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
P2 CA-137 1e5 96% 99% Plasmid (general) 0.4 µg 20 µl 4D X-Unit

Citations

An engineered human cardiac tissue model reveals contributions of systemic lupus erythematosus autoantibodies to myocardial injury
Categories:
Primary Cells and Media 
Authors:
Sharon Fleischer, Trevor R. Nash, Manuel A. Tamargo, Roberta I. Lock, Gabriela Venturini, Margaretha Morsink, Pamela L. Graney, Vanessa Li, Morgan J. Lamberti, Martin Liberman, Youngbin Kim, Daniel N. Tavakol, Richard Z. Zhuang, Jaron Whitehead, Richard A. Friedman, Rajesh K. Soni, Jonathan G. Seidman, Christine E. Seidman, Laura Geraldino-Pardilla, Robert Winchester & Gordana Vunjak-Novakovic  
In:
Nature (2024) 3: 9 
Egr-1 upregulates Siva-1 expression and induces cardiac fibroblast apoptosis
Categories:
Primary Cells and Media, Serum-free and Speciality Media 
Authors:
Zins K1, Pomyje J2, Hofer E3, Abraham D4, Lucas T5, Aharinejad S6. 
In:
Int J Mol Sci (2014) 15(1): 1538-53 
Cenderitide-eluting film for potential cardiac patch applications.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Ng XW, Huang Y, Chen HH, Burnett JC Jr, Boey FY, Venkatraman SS. 
In:
PLoS ONE (2013) 8(7): e68346 

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