Fibroblast, lung (DHFL), human asthma

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Asthma is a chronic lung disease that inflames and narrows the airways. Asthma causes recurring periods of wheezing (a whistling sound when you breathe), chest tightness, shortness of breath, and coughing.

Cell Type:
Fibroblast
Tissue Origin:
lung
Species:
human
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Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent

Recommended Media

Fibroblast Growth Medium-2

Fibroblast media has been optimized for the proliferation of fibroblasts.
FGM-2 contains a vial of FBS for a final serum concentration of 2%

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
P3 CA-158 1e5 78% ±1 79% ±6 Plasmid (general) 0.4 µg 20 µl 4D X-Unit
P3 CA-158 5e5 71% 74% ±2 Plasmid (general) 2 µg 100 µl 4D X-Unit

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