cells

Asthma is a chronic lung disease that inflames and narrows the airways. Asthma causes recurring periods of wheezing (a whistling sound when you breathe), chest tightness, shortness of breath, and coughing.

Smooth muscle cells isolated from the major bronchia.

Cell Type:

Smooth muscle cell

Tissue Origin:

lung

Species:

human

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Research Area:

Cancer Research/Cell Biology

Cell Characteristics:

Adherent

Recommended Media

Smooth Muscle Growth Medium-2

SmGM-2 was developed for the maintenance and expansion of human smooth muscle cells. This medium contains 5% FBS.

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Protocol	Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
	P1	CA-137	1e5	83% ±1	64% ±3	Plasmid (general)	0.2 µg	20 µl	4D X-Unit
	P1	CA-137	5e5	81% ±1	52% ±1	Plasmid (general)	1 µg	100 µl	4D X-Unit

SMC, bronchial (DBSMC), human asthma

Recommended Media

Transfection Information