Epithelial, bronchial (DHBE), human asth

Asthma is a chronic lung disease that inflames and narrows the airways. Asthma causes recurring periods of wheezing (a whistling sound when you breathe), chest tightness, shortness of breath, and coughing.

Human bronchial/tracheal epithelial cells are isolated from the epithelial cells that line the airway above the bifurcation of the lungs.

Cell Type:
Respiratory Tract Epith.
Tissue Origin:
lung
Species:
human
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent

Recommended Media

Airway cell media are serum-free media that have been optimized for the proliferation of airway cells.

BEGM is optimized to provide the best growth for Normal Human Bronchial/Tracheal Epithelial Cells.

Media developed specifically for cultivation of bronchial epithelial cells in an air-liquid interface system.

The B-ALI™ BulletKit includes:
 - B-ALI™ Growth Basal Medium (200 ml)
 - B-ALI™ Differentiation Basal Medium (500 ml)
 - B-ALI™ SingleQuots Kit

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
P3 DN-100 1e5 73% 78.5% Plasmid (general) 0.4 µg 20 µl Shuttle
P3 DC-100 1e5 41.5% 77.3% Plasmid (general) 0.4 µg 20 µl Shuttle
P3 DC-100 5e5 60.7% ± 69.7% ±1 Plasmid (general) 2 µg 100 µl 4D X-Unit
P3 DC-100 1e5 56.1% ±1 80.3% ±1 Plasmid (general) 0.4 µg 20 µl 4D X-Unit

Citations

Categories:
Primary Cells and Media 
Authors:
Park JA, Kim JH, Bi D, Mitchel JA, Qazvini NT, Tantisira K, Park CY, McGill M, Kim SH, Gweon B, Notbohm J, Steward R Jr, Burger S, Randell SH, Kho AT, Tambe DT, Hardin C, Shore SA, Israel E, Weitz DA, Tschumperlin DJ, Henske EP, Weiss ST, Manning ML, Butler JP, Drazen JM, Fredberg JJ 
In:
Other (2015) 14(10): 1040-8 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Nino G, Huseni S, Perez GF, Pancham K, Mubeen H, Abbasi A, Wang J, Eng S, Colberg-Poley AM, Pillai DK, Rose MC 
In:
PLoS ONE (2014) 9(12): e115398 
Categories:
Primary Cells and Media 
Authors:
Pennino D, Bhavsar PK, Effner R, Avitabile S, Venn P, Quaranta M, Marzaioli V, Cifuentes L, Durham SR, Cavani A, Eyerich K, Chung KF, Schmidt-Weber CB, Eyerich S. 
In:
J Allergy Clin Immunol (2013) 131(2): 562-70