Epithelial, bronchial (DHBE), human COPD

Chronic obstructive pulmonary disease, is a progressive disease that makes it hard to breathe. Chronic bronchitis & emphysema are other names/stages of COPD. COPD can cause coughing that produces large amounts of mucus, wheezing, shortness of breath, chest tightness, and other symptoms.

Human bronchial/tracheal epithelial cells isolated from the epithelial cells that line the airway above the bifurcation of the lungs.

Cell Type:
Respiratory Tract Epith.
Tissue Origin:
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:

Recommended Media

Airway cell media are serum-free media that have been optimized for the proliferation of airway cells.

BEGM is optimized to provide the best growth for Normal Human Bronchial/Tracheal Epithelial Cells.

Media developed specifically for cultivation of bronchial epithelial cells in an air-liquid interface system.

The B-ALI™ BulletKit includes:
 - B-ALI™ Growth Basal Medium (200 ml)
 - B-ALI™ Differentiation Basal Medium (500 ml)
 - B-ALI™ SingleQuots Kit

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
P3 DC-100 1e5 57.1% ±1 76.7% ± Plasmid (general) 0.6 µg 20 µl 4D X-Unit
P3 DC-100 5e5 76.5% ± 69.9% ±2 Plasmid (general) 3 µg 100 µl 4D X-Unit


Primary Cells and Media, 3D Cell Culture , Transfection 
Radu Rapiteanu??1, Tina Karagyozova1, Natalie Zimmermann2, Gareth Wayne1, MatteoMartufi1, Nikolai N Belyaev2, Kuljit Singh1, Joanna Betts1, Soren Beinke2 and Klio Maratou1 
BioResearch Open Access (2019) 01: 01