SMC, bronchial, human normal (BSMC)

Smooth muscle cells isolated from the major bronchia.

Cell Type:
Smooth muscle cell
Tissue Origin:
lung
Species:
human
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent

Recommended Media

 SmGM-2 was developed for the maintenance and expansion of human smooth muscle cells. This medium contains 5% FBS.

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
P1 CA-137 1e5 65% 63% Plasmid (general) 0.2 µg 20 µl 4D X-Unit
P1 CA-137 5e5 76% 71% Plasmid (general) 1 µg 100 µl 4D X-Unit

Citations

Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Calvén J, Yudina Y, Uller L 
In:
PLoS ONE (2013) 8(4): e62718 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
May RD, Monk PD, Cohen ES, Manuel D, Dempsey F, Davis NH, Dodd AJ, Corkill DJ, Woods J, Joberty-Candotti C, Conroy LA, Koentgen F, Martin EC, Wilson R, Brennan N, Powell J, Anderson IK 
In:
Br J Pharmacol (2012) 166(1): 177-93 
Categories:
Primary Cells and Media 
Authors:
Yamamoto Y, Takase K, Kishino J, Fujita M, Okamura N, Sakaeda T, Fujimoto M, Yagami T 
In:
PLoS ONE (2011) 6(3): e17552 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Yagami A, Orihara K, Morita H, Futamura K, Hashimoto N, Matsumoto K, Saito H, Matsuda A. 
In:
J Immunol (2010) 185(10): 5743-50 
Categories:
Primary Cells and Media 
Authors:
Kuhn AR, Schlauch K, Lao R, Halayko AJ, Gerthoffer WT, Singer CA 
In:
Am J Respir Cell Mol Biol (2010) 42(4): 506-13 
Categories:
Primary Cells and Media 
Authors:
Erlemann KR, Cossette C, Gravel S, Lesimple A, Lee GJ, Saha G, Rokach J, Powell WS 
In:
Free Radic Biol Med (2007) 42(5): 654-64