cells

Madin-Darby canine kidney

Cell Type:

Kidney Epith.

Tissue Origin:

kidney

Species:

canine

Cell Characteristics:

Adherent

Recommended Media

UltraMDCK

UltraMDCK Serum-free Renal Cell Medium is designed to support the growth of MADIN-DARBY Canine Kidney (MDCK) cells at low and high plating densities.
The medium contains only two proteins: recombinant human insulin and bovine transferrin, yielding a very low protein formulation.

Storage = 2ºC to 8ºC

ProVero 1

ProVero™ 1 Serum-free Medium is a protein-free medium designed to support the growth of MDCK and vero cells. It contains HEPES and sodium bicarbonate buffer.
The absence of proteins and only very low levels of human recombinant insulin facilitate both downstream processing and regulatory compliance.

Some Vero cell strains require additional supplementation with 5.0 µg/L rhEGF for optimal vero cell growth.

Storage = 2°C to 8°C

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
L	A-024	5e5	69-77%	79-87%	Plasmid (general)	5 µg	100 µl	I/II/2b
SE	CA-152	1e6	87-89%	50-64%	Plasmid (general)	5 µg	100 µl	4D X-Unit
SE	CA-152	2e5	81-85%	41-61%	Plasmid (general)	1 µg	20 µl	4D X-Unit
SF	FF-150	2e5	90-99%	90-99%	Plasmid (general)	200 ng	20 µl	96-well
SF	FF-138	2e5	80-90%	80-90%	Plasmid (general)	200 ng	20 µl	96-well

Citations (20)

Clathrin is a key regulator of basolateral polarity

Categories:

Transfection

Authors:

Deborde S, Perret E, Gravotta D, Deora A, Salvarezza S, Schreiner R, Rodriguez-Boulan E

In:

Nature (2008) 452(7188): 719-23

Involvement of Rab13 and JRAB/MICAL-L2 in epithelial cell scattering

Categories:

Transfection

Authors:

Kanda I, Nishimura N, Nakatsuji H, Yamamura R, Nakanishi H, Sasaki T

In:

Oncogene (2008) 27(12): 1687-95

Involvement of nectin in inactivation of integrin alpha vbeta 3 after the establishment of cell-cell adhesion

Categories:

Transfection

Authors:

Sakamoto Y, Ogita H, Komura H, Takai Y

In:

J Biol Chem (2008) 283(1): 496-505

Polarity proteins PAR6 and aPKC regulate cell death through GSK-3beta in 3D epithelial morphogenesis

Categories:

Transfection

Authors:

Kim M, Datta A, Brakeman P, Yu W, Mostov KE

In:

J Cell Sci (2007) 120(Pt 14): 2309-17

Full-length, glycosylated NSP4 is localized to plasma membrane caveolae by a novel raft isolation technique

Categories:

Transfection

Authors:

Storey SM, Gibbons TF, Williams CV, Parr RD, Schroeder F, Ball JM

In:

J Virol (2007) 81(11): 5472-83

Par1b Promotes Hepatic-type Lumen Polarity in MDCK Cells via Myosin II and E-Cadherin-dependent Signaling

Categories:

Transfection

Authors:

Cohen D, Tian Y, Musch A

In:

Mol Biol Cell (2007) 18(6): 2203-15

Involvement of integrin-induced activation of protein kinase C in the formation of adherens junctions

Categories:

Transfection

Authors:

Ozaki M, Ogita H, Takai Y

In:

Genes Cells (2007) 12(5): 651-62

Acute regulation of the urea transporter mUT-A3 expressed in a MDCK cell line

Categories:

Transfection

Authors:

Stewart GS, King SL, Potter EA, Smith CP

In:

Am J Physiol Renal Physiol (2007) 292(4): F1157-63

Prominin-2 is a cholesterol-binding protein associated with apical and basolateral plasmalemmal protrusions in polarized epithelial cells and released into urine

Categories:

Transfection

Authors:

Florek M, Bauer N, Janich P, Wilsch-Braeuninger M, Fargeas CA, Marzesco AM, Ehninger G, Thiele C, Huttner WB, Corbeil D

In:

Cell Tissue Res (2007) 328(1): 31-47

Depletion of E-Cadherin Disrupts Establishment but Not Maintenance of Cell Junctions in MDCK Epithelial Cells

Categories:

Transfection

Authors:

Capaldo CT, Macara IG

In:

Mol Biol Cell (2007) 18(1): 189-200

FAPP2, cilium formation, and compartmentalization of the apical membrane in polarized Madin-Darby canine kidney (MDCK) cells

Categories:

Transfection

Authors:

Vieira OV, Gaus K, Verkade P, Fullekrug J, Vaz WL, Simons K

In:

Proc Natl Acad Sci USA (2006) 103(49): 18556-61

Requirement of nectin, but not cadherin, for formation of claudin-based tight junctions in annexin II-knockdown MDCK cells

Categories:

Transfection

Authors:

Yamada A, Fujita N, Sato T, Okamoto R, Ooshio T, Hirota T, Morimoto K, Irie K, Takai Y

In:

Oncogene (2006) 25(37): 5085-102

PAR1b Promotes Cell-Cell Adhesion and Inhibits Dishevelled-mediated Transformation of MDCK Cells

Categories:

Transfection

Authors:

Elbert M, Cohen D, Musch A

In:

Mol Biol Cell (2006) 17(8): 3345-55

Urea flux across MDCK-mUT-A2 monolayers is acutely sensitive to AVP, cAMP and [Ca2+]i

Categories:

Transfection

Authors:

Potter EA, Stewert G, Smith CP

In:

Am J Physiol Renal Physiol (2006) 291(1): F122-8

Zonula occludens-1 function in the assembly of tight junctions in madin-darby canine kidney epithelial cells

Categories:

Transfection

Authors:

McNeil E, Capaldo C and Macara IG

In:

Mol Biol Cell (2006) 17(4): 1922-32

Regulation of the assembly and adhesion activity of E-cadherin by nectin and afadin for the formation of adherens junctions in MDCK cells

Categories:

Transfection

Authors:

Sato T, Fujita N, Yamada A, Ooshio T, Okamoto R, Irie K and Takai Y

In:

J Biol Chem (2006) 281(8): 5288-99

Par-3 mediates the inhibition of LIM kinase 2 to regulate cofilin phosphorylation and tight junction assembly

Categories:

Transfection

Authors:

Chen X, Macara IG

In:

J Cell Biol (2006) 172(5): 671-8

Phase coexistence and connectivity in the apical membrane of polarized epithelial cells

Categories:

Transfection

Authors:

Meder D, Moreno MJ, Verkade P, Vaz WL and Simons K

In:

Proc Natl Acad Sci USA (2006) 103(2): 329-334

RNA interference techniques to study epithelial cell adhesion and polarity

Categories:

Transfection

Authors:

Chen X, Macara IG

In:

Methods Enzymol (2006) 406: 362-74

Inversin, the gene product mutated in nephronophthisis type II, functions as a molecular switch between Wnt signaling pathways

Categories:

Transfection

Authors:

Simons M, Gloy J, Ganner A, Bullerkotte A, Bashkurov M, Kronig C, Schermer B, Benzing T, Cabello OA, Jenny A, Mlodzik M, Polok B, Driever W, Obara T and Walz G

In:

Nat Genet (2005) 37(5): 537-543

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