Embryonic fibroblast, mouse (MEF)primary

Primary (non-immortalized) embryonic fibroblasts from mouse.

Cell Type:
Fibroblast
Tissue Origin:
embryo
Species:
mouse
Research Area:
Dermatology/Tissue Engineering
Stem Cells
Cell Characteristics:
Adherent

Recommended Media

Dulbecco's Modified Eagle Medium (DMEM) was developed in 1969 and is a modification of Basal Medium Eagle (BME) that differs from BME and MEM by the following characteristics:

  • Vitamins 4X greater than MEM. Vitamins and amino acids greater than BME
  • Types and quantities of amino acids greater than MEM and BME
  • Iron (ferric nitrate)

It is used in a wide range of mammalian cell culture applications.  The high glucose version is well suited to high density suspension culture. The low glucose formula is used for adherent dependent cells.

Storage = 2ºC to 8ºC

Liquid:
12-614 = 4.5 g/L glucose, without L-glutamine
12-604 = 4.5 g/L glucose, with L-glutamine
BE12-604/U1 = 4.5 g/L glucose, with Ultraglutamine I
12-914 = 4.5 g/L glucose, without L-glutamine (hybridoma screened)
12-917 = 4.5 g/L glucose, without L-glutamine or phenol red
12-709 = 4.5 g/L glucose and 25 mM HEPES, without L-glutamine
12-733= 4.5 g/L glucose, without L-glutamine or sodium pyruvate
12-741 = 4.5 g/L glucose, with L-glutamine, without sodium pyruvate

12-707 = 1.0 g/L glucose, without L-glutamine
12-708 = 1.0 g/L glucose and 25 mM HEPES, without L-glutamine

Powder:
15-614 = 4.5 g/L glucose, without L-glutamine or sodium pyruvate
15-604 = 4.5 g/L glucose, with L-glutamine or sodium pyruvate

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
P4 CZ-167 1e5 67% 86% Plasmid (general) 400 ng 20 µl 4D X-Unit
P4 CZ-167 5e5 68% 90% Plasmid (general) 2 µg 100 µl 4D X-Unit
P4 CZ-167 1e5 89% 75% Plasmid (general) 400 ng 20 µl Shuttle
Hepatocyte, mouse/rat N-024 2e6 90% 78% Plasmid (general) 2 µg 100 µl I/II/2b
AD2 FB-166 25% good Plasmid (general) 16 µg 350 µl 4D Y-Unit

Citations (10)

Categories:
Primary Cells and Media 
Authors:
Kaneda MM, Cappello P, Nguyen AV, Ralainirina N, Hardamon CR, Foubert P, Schmid MC, Sun P, Mose E, Bouvet M, Lowy AM, Valasek MA, Sasik R, Novelli F, Hirsch E, Varner JA 
In:
Cancers (2016) 6(8): 870-885 
Categories:
Primary Cells and Media 
Authors:
Kolb AW, Lee K, Larsen I, Craven M, Brandt CR 
In:
PLoS Pathog (2016) 12(3): ePub 
Categories:
Transfection 
Authors:
Cynthia R. Coffill, Alison P. Lee, Jia Wei Siau, Sharon M. Chee, Thomas L. Joseph, Yaw Sing Tan, Arumugam Madhumalar, Boon-Hui Tay, Sydney Brenner, Chandra S. Verma, Farid J. Ghadessy, Byrappa Venkatesh, and David P. Lane 
In:
Genes Dev (2016) 30(3): 281-92 
Categories:
Primary Cells and Media, Transfection 
Authors:
Kim SM, Kim JW, Kwak TH, Park SW, Kim KP, Park H, Lim KT, Kang K, Kim J, Yang JH, Han H, Lee I, Hyun JK, Bae YM, Schöler HR, Lee HT, Han DW. 
In:
J Biol Chem (2016) 291: 27 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Jank BJ, Xiong L, Moser PT, Guyette JP, Ren X, Cetrulo CL, Leonard DA, Fernandez L, Fagan SP, Ott HC 
In:
Biomaterials (2015) 61: 246-56 
Categories:
 
Authors:
Valentine CD, Zhang H, Phuan PW, Nguyen J, Verkman AS, Haggie PM. 
In:
Cell Microbiol (2014) 16(1): 1-14 
Categories:
 
Authors:
You F, Wang P, Yang L, Yang G, Zhao YO, Qian F, Walker W, Sutton R, Montgomery R, Lin R, Iwasaki A, Fikrig E. 
In:
Nat Immunol (2013) 14(12): 1237-46 
Categories:
Primary Cells and Media, Transfection 
Authors:
Martins M, Warren S, Kimberley C, Margineanu A, Peschard P, McCarthy A, Yeo M, Marshall CJ, Dunsby C, French PM, Katan M. 
In:
J Cell Sci (2012) 125(23): 5758-69 
Categories:
 
Authors:
Tulloch NL, Muskheli V, Razumova MV, Korte FS, Regnier M, Hauch KD, Pabon L, Reinecke H, Murry CE. 
In:
Circ Res (2011) 109(1): 47-59 
Categories:
Primary Cells and Media, Transfection 
Authors:
Bernot D, Barruet E, Poggi M, Bonardo B, Alessi MC, Peiretti F. 
In:
J Biol Chem (2010) 285(9): 6508-14 
+ Show All