melanoma cell line, human (RIPA)
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melanoma cell line, human (RIPA)
Cell Type:
Skin/Hair Epith.
Tissue Origin:
Species:
human
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
SE
EH-100
1e5
40%
70%
Plasmid (general)
0.4 µg
4D X-Unit
SE
EN-150
1e5
30%
75%
Plasmid (general)
0.4 µg
4D X-Unit
Citations
Metabolism of melatonin and biological activity of intermediates of melatoninergic pathway in human skin cells
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media
Authors:
Kim TK1, Kleszczynski K, Janjetovic Z, Sweatman T, Lin Z, Li W, Reiter RJ, Fischer TW, Slominski AT.
In:
FASEB J (2013) 27(7): 2742-55
Identification of aurora kinase B and Wee1-like protein kinase as downstream targets of (V600E)B-RAF in melanoma.
Categories:
Transfection
Authors:
Sharma A, Madhunapantula SV, Gowda R, Berg A, Neves RI, Robertson GP.
In:
Am J Pathol (2013) 182(4): 1151-62