cells

fibroblast

Cell Type:

Fibroblast

Tissue Origin:

dermal

Species:

porcine

Research Area:

Cancer Research/Cell Biology

Dermatology/Tissue Engineering

Cell Characteristics:

Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
P3	EN-150	1e5	90%	90%	Plasmid (general)	0.4 µg	20 µl	96-well
Fibroblast, basic	U-023	1e6	78-80%	60%	Plasmid (general)	10 µg	100 µl	I/II/2b
Fibroblast, basic	A-033	1e6	75-78%	62-63%	Plasmid (general)	10 µg	100 µl	I/II/2b
P3	EH-100	1e5	37%	85.5%	Plasmid (general)	0.4 µg	20 µl	4D X-Unit
P3	CM-138	1e5	33.1%	88.6%	Plasmid (general)	0.4 µg	20 µl	4D X-Unit

Citations

Establishment of Induced Pluripotent Stem Cells from Fishing Cat and Clouded Leopard Using Integration-Free Method for Wildlife Conservation

Categories:

Primary Cells and Media, Transfection

Authors:

W. Sukparangsi A , R. Bootsri A , W. Sikeao A , S. Karoon B and A. Thongphakdee B

In:

Reprod Sci (2017) 30(1): 230

Production of Mutated Porcine Embryos Using Zinc Finger Nucleases and a Reporter-based Cell Enrichment System.

Categories:

Transfection

Authors:

Koo OJ, Park SJ, Lee C, Kang JT, Kim S, Moon JH, Choi JY, Kim H, Jang G, Kim JS, Kim S, Lee BC.

In:

Asian J Androl (2014) 27(3): 324-9

Transgene expression of green fluorescent protein and germ line transmission in cloned pigs derived from in vitro transfected adult fibroblasts.

Categories:

Transfection

Authors:

Brunetti D1, Perota A, Lagutina I, Colleoni S, Duchi R, Calabrese F, Seveso M, Cozzi E, Lazzari G, Lucchini F, Galli C.

In:

Stem Cells (2008) 10(4): 409(19)

Efficient transfection of primarily cultured porcine embryonic fibroblasts using the Amaxa Nucleofection system.

Categories:

Transfection

Authors:

Nakayama A1, Sato M, Shinohara M, Matsubara S, Yokomine T, Akasaka E, Yoshida M, Takao S.

In:

Stem Cells (2007) 9(4): 523(34

Fibroblast, pig

Transfection Information

Citations