NRK52E

kidney epithelial-like cell line

Cell Type:
Kidney Epith.
Tissue Origin:
kidney
Species:
rat
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent

Recommended Media

PC-1™ is a low-protein, serum-free medium intended for the culture of primary cells and anchorage-dependent cell lines. PC-1 is formulated in a specially modified DMEM/F12 base and contains a complete HEPES buffering system with known amounts of insulin, transferrin, fatty acids, and proprietary proteins assembled under strict quality control procedures. PC-1™ is intended for a variety of research and industrial applications and is formulated using defined components for optimal cell growth, while maintaining the lowest possible protein content.
PC-1™ does not contain L-glutamine.

PC-1™ Liquid Base Medium is to be stored at 2°C-8°C.
The Supplement should be stored at -20°C.
When these two components are combined, the resulting PC-1™ Complete Medium is stable for 45 days at 2°C-8°C.

Once thawed, the appropriate volume of one vial of PC-1™ Supplement must be combined with the companion volume of PC-1™ Liquid Base Medium. Partial reconstitution or repeated freezing and thawing of the PC-1™ Supplement is not advised.

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
V T-027 2e6 90% 50% 5 µg 100 µl I/II/2b

Citations

Categories:
Transfection 
Authors:
Zhou M, Wang YL 
In:
Mol Biol Cell (2008) 19(1): 318-26 
Categories:
Transfection 
Authors:
Tanoue T and Takeichi M 
In:
J Cell Biol (2004) 165(4): 517-528