human renal cell adenocarcinoma derived from metastatic site: pleural effusion

Cell Type:
GI Tract Epith.
Tissue Origin:
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:

Recommended Media

RPMI-1640 medium was developed by Moore et al., at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI-1640 medium has been used for the culture of human normal and neoplastic leukocytes. RPMI-1640 when properly supplemented, has demonstrated wide applicability for supporting growth of many types of cell cultures, including fresh human lymphocytes in the 72-hour phytohemagglutinin (PHA) stimulation assay.

There are a variety of formulations:
12-702         - with L-glutamine
BE12-702/U1 - with UltraGlutamineI
BE15-702D    - [powder] with L-glutamine
12-167          - without L-glutamine
12-115          - with L-glutamine and 25 mM HEPES buffer
BE12-115/U1 - with UltraGlutamineI and 25 mM HEPES buffer
04-525          - with L-glutamine and 165 nM MOPS (used for some mycological assays)
09-774          - with L-glutamine, 25 mM HEPES buffer 100 units/ml penicillin, 50 ug/ml streptomycin
12-918          - without L-glutamine or phenol red
BE12-752       - with L-glutamine, without D-glucose

Storage = 2ºC to 8ºC 
  (versions without L-glutamine and those with UltraGlutamine can be stored at 15ºC to 30ºC)

MEM Eagle (also called EMEM) was developed in 1959 for cultivation of HeLa and L cells. Amino acid concentrations conform closely to the protein composition of human cells. Higher concentrations of nutrients permit longer periods between feedings. EMEM has vitamin concentrations 2-5X greater than BME and higher amino acid concentrations than BME. EMEM is suitable for culturing a broad spectrum of mammalian cells.

MEM Eagle is available with Hanks' or Earle's salts.
MEM-Hanks (12-127F or 12-137F) does not a require CO2 enriched atmosphere. 
Joklik's modification is intended for suspension culture.

Storage = 2ºC to 8ºC
                (or 15-30ºC for products 12-125, 12-136, 12-684, 12-668, 12-127 and 12-137)

MEM Eagle is available in several variations:
 - with Earle's BSS
12-611 - with L-glutamine
12-125 - without L-glutamine
12-622 - with non-essential amino acids (NEAA), and sodium pyruvate, without L-glutamine
12-136 - with L-glutamine and HEPES
12-736 - with L-glutamine, HEPES, NEAA, gentamycin, pen/strep, ampB and 2% FBS
12-684 - 10x media without sodium bicarbonate or L-glutamine
12-668 - 2x media without L-glutamine or phenol red (virus plaquing medium)
06-174 - without calcium, with NEAA and L-glutamine

- with Hank's BSS
12-127 - without L-glutamine
12-137 - with HEPES, without L-glutamine

- Joklik's Formulation

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
V T-030 1e6 85% 60% Plasmid (general) 2 µg 100 µl I/II/2b


M Kuhlwilm, A Davierwala, S Pääbo 
PLoS ONE (2013) 8 (12): e83218