human renal cell adenocarcinoma derived from metastatic site: pleural effusion
RPMI-1640 medium was developed by Moore et al., at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI-1640 medium has been used for the culture of human normal and neoplastic leukocytes. RPMI-1640 when properly supplemented, has demonstrated wide applicability for supporting growth of many types of cell cultures, including fresh human lymphocytes in the 72-hour phytohemagglutinin (PHA) stimulation assay.There are a variety of formulations:12-702 - with L-glutamineBE12-702/U1 - with UltraGlutamineIBE15-702D - [powder] with L-glutamine12-167 - without L-glutamine12-115 - with L-glutamine and 25 mM HEPES bufferBE12-115/U1 - with UltraGlutamineI and 25 mM HEPES buffer04-525 - with L-glutamine and 165 nM MOPS (used for some mycological assays)09-774 - with L-glutamine, 25 mM HEPES buffer 100 units/ml penicillin, 50 ug/ml streptomycin12-918 - without L-glutamine or phenol redBE12-752 - with L-glutamine, without D-glucoseStorage = 2ºC to 8ºC (versions without L-glutamine and those with UltraGlutamine can be stored at 15ºC to 30ºC)
MEM Eagle (also called EMEM) was developed in 1959 for cultivation of HeLa and L cells. Amino acid concentrations conform closely to the protein composition of human cells. Higher concentrations of nutrients permit longer periods between feedings. EMEM has vitamin concentrations 2-5X greater than BME and higher amino acid concentrations than BME. EMEM is suitable for culturing a broad spectrum of mammalian cells.MEM Eagle is available with Hanks' or Earle's salts.MEM-Hanks (12-127F or 12-137F) does not a require CO2 enriched atmosphere. Joklik's modification is intended for suspension culture.Storage = 2ºC to 8ºC (or 15-30ºC for products 12-125, 12-136, 12-684, 12-668, 12-127 and 12-137)MEM Eagle is available in several variations: - with Earle's BSS12-611 - with L-glutamine12-125 - without L-glutamine12-622 - with non-essential amino acids (NEAA), and sodium pyruvate, without L-glutamine12-136 - with L-glutamine and HEPES12-736 - with L-glutamine, HEPES, NEAA, gentamycin, pen/strep, ampB and 2% FBS12-684 - 10x media without sodium bicarbonate or L-glutamine12-668 - 2x media without L-glutamine or phenol red (virus plaquing medium)06-174 - without calcium, with NEAA and L-glutamine- with Hank's BSS12-127 - without L-glutamine12-137 - with HEPES, without L-glutamine- Joklik's Formulation04-719
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