cells

Hybrid cell line formed by the electrofusion of a primary culture of NEDH rat pancreatic islets with RINm5F (a cell line derived from a NEDH rat insuliinoma), resulting in a stable glucose-responsive insulin-secreting beta-cell line

Cell Type:

Pancreas

Tissue Origin:

pancreas

Species:

rat

Research Area:

Cancer Research/Cell Biology

Gastroenterology

Cell Characteristics:

Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Protocol	Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
	V	X-001	2e5	40-50%	90%	Plasmid (general)	2 µg	100 µl	I/II/2b

BRIN-BD11

Transfection Information