NKL
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NK cell line, established from patient with CD3-, CD16+,CD56+, large granulocyte leukemia
Cell Type:
Natural Killer (NK)
Tissue Origin:
blood
Species:
human
Research Area:
Immunotherapy / Hematology
Cell Characteristics:
Suspension
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
V
O-017
2e6
40-70%
90%
Plasmid (general)
2 µg
100 µl
I/II/2b
Citations (7)
JNK MAP kinase activation is required for MTOC and granule polarization in NKG2D-mediated NK cell cytotoxicity
Categories:
Transfection
Authors:
Li C, Ge B, Nicotra M, Stern JN, Kopcow HD, Chen X, Strominger JL
In:
Proc Natl Acad Sci USA (2008) 105(8): 3017-22
Regulation of human DAP10 gene expression in NK and T cells by Ap-1 transcription factors
Categories:
Transfection
Authors:
Marusina AI, Burgess SJ, Pathmanathan I, Borrego F, Coligan JE
In:
J Immunol (2008) 180(1): 409-17
Identification, activation, and selective in vivo ablation of mouse NK cells via NKp46
Categories:
Transfection
Authors:
Walzer T, Blery M, Chaix J, Fuseri N, Chasson L, Robbins SH, Jaeger S, Andre P, Gauthier L, Daniel L, Chemin K, Morel Y, Dalod M, Imbert J, Pierres M, Moretta A, Romagne F, Vivier E
In:
Proc Natl Acad Sci USA (2007) 104(9): 3384-9
IL-21 Down-Regulates NKG2D/DAP10 Expression on Human NK and CD8+ T Cells
Categories:
Transfection
Authors:
Burgess SJ, Marusina AI, Pathmanathan I, Borrego F and Coligan JE
In:
J Immunol (2006) 176(3): 1490-1497
GATA-3 Is an Important Transcription Factor for Regulating Human NKG2A Gene Expression
Categories:
Transfection
Authors:
Marusina AI, Kim DK, Lieto LD, Borrego F and Coligan JE
In:
J Immunol (2005) 174(4): 2152-2159
Efficient gene transfer into the human natural killer cell line, NKL, using the amaxa nucleofection system
Categories:
Transfection
Authors:
Maasho K, Marusina A, Reynolds NM, Coligan JE and Borrego F
In:
J Immunol Methods (2004) 284: 133-140
Rapid and highly efficient gene transfer into natural killer cells by nucleofection
Categories:
Transfection
Authors:
Trompeter HI, Weinhold S, Thiel C, Wernet P and Uhrberg M
In:
J Immunol Methods (2003) 274: 245-256
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