cells

Gestational choriocarcinoma cell line derived from metastatic site: Brain.

Cell Type:

Embryonic/Extraembryonic Epith.

Tissue Origin:

Species:

human

Research Area:

Cancer Research/Cell Biology

Cell Characteristics:

Adherent

Recommended Media

DMEM:F12

DMEM:F12 combines the richness of F12 with the higher component concentrations of DMEM. This medium is well suited for clonal density cultures and has been extensively used to demonstrate the effect of various hormones and growth factors on target tissues.

Storage = 2ºC to 8ºC

12-719 = 3.151 g/L glucose, L-glutamine and 15mM HEPES
BE04-687Q = 3.151 g/L glucose and L-glutamine, without HEPES
BE04-687F/U1 = 3.151 g/L glucose and Ultraglutamine I, without HEPES

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Protocol	Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
	L	X-005	1e6	90%	85%	siRNA		100 µl	I/II/2b

Citations

Methods for siRNA-mediated Reduction of mRNA and Protein Expression in Human Placental Explants, Isolated Primary Cells and Cell Lines

Categories:

Transfection

Authors:

K. Forbes, M. Desforges, R. Garside, J.D. Aplin, and M. Westwood

In:

Placenta (2009) 30(2): 124-129

Methods for siRNA-mediated reduction of mRNA and protein expression in human placental explants, isolated primary cells and cell lines.