Endothelial, MV dermal, human adult

Endothelial cells line the inside surface of blood vessels, heart, lymphatic vessels, body cavities and other organs, of normal human tissue.

Cell Type:
Microvascular Endo.
Tissue Origin:
dermal
Species:
human
Research Area:
Cancer Research/Cell Biology
Cardiovascular
Dermatology/Tissue Engineering
Cell Characteristics:
Adherent

Recommended Media

EGM-2 MV has been developed for the enhanced growth of lung microvascular endothelial cells
Can be used to grow all Clonetics Endothelial cells except HUVEC
Final serum concentration is 5%
Does not contain BBE

This medium has been discontinued end of 2023. As alternative we recommend the EGM-2 MV CC-3202

Developed for microvascular and coronary artery endothelial cells
Same basal medium as in EGM
Final serum concentration is 5%
EGM-MV can be used to grow most Clonetics Endothelial Cells

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Endothelial, basic M-003 4e5 64% ±6 99% ±5 Plasmid (general) 2 µg 100 µl I/II/2b
Endothelial, basic T-005 4e5 64% ±3 97% ±2 Plasmid (general) 2 µg 100 µl I/II/2b

Citations (7)

Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Sharma-Walia N, Chandran K, Patel K, Veettil MV, Marginean A. 
In:
J Virol (2014) 88(4): 2131-56 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Miklas JW, Dallabrida SM, Reis LA, Ismail N, Rupnick M, Radisic M. 
In:
PLoS ONE (2013) 8(8): e72956 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Bottero V1, Chakraborty S, Chandran B. 
In:
J Virol (2013) 87(3): 1733-49 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Yasuniwa Y, Izumi H, Wang KY, Shimajiri S, Sasaguri Y, Kawai K, Kasai H, Shimada T, Miyake K, Kashiwagi E, Hirano G, Kidani A, Akiyama M, Han B, Wu Y, Ieiri I, Higuchi S, Kohno K. 
In:
PLoS ONE (2010) 5(12): e15330 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Efklidou S, Bailey R, Field N, Noursadeghi M, Collins MK. 
In:
J Cell Sci (2008) 121(Pt 4): 450-7 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Clarke PA, Dickson JH, Harris JC, Grabowska A, Watson SA. 
In:
Cancer Res (2006) 66(7): 3504-12 
Categories:
Primary Cells and Media 
Authors:
Chi JT, Chang HY, Haraldsen G, Jahnsen FL, Troyanskaya OG, Chang DS, Wang Z, Rockson SG, van de Rijn M, Botstein D, Brown PO. 
In:
Proc Natl Acad Sci USA (2003) 100 (19): 10623-8 
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