Endothelial, MV dermal, human neo

Human Dermal Microvascular Endothelial Cells from neonatal donor (HMVEC-d Neo). Endothelial cells from the inside surface of blood vessels, heart, lymphatic vessels, body cavities and other organs, of normal human tissue. Small vessel endothelial cells are isolated from dermal microvascular tissue.

Cell Type:
Microvascular Endo.
Tissue Origin:
dermal
Species:
human
Research Area:
Cancer Research/Cell Biology
Cardiovascular
Dermatology/Tissue Engineering
Cell Characteristics:
Adherent

Recommended Media

EGM-2 MV has been developed for the enhanced growth of lung microvascular endothelial cells
Can be used to grow all Clonetics Endothelial cells except HUVEC
Final serum concentration is 5%
Does not contain BBE

Developed for microvascular and coronary artery endothelial cells
Same basal medium as in EGM
Final serum concentration is 5%
EGM-MV can be used to grow most Clonetics Endothelial Cells

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Endothelial, basic T-023 5e6 90% good Plasmid (general) 2 µg 100 µl I/II/2b
Endothelial, basic M-003 4e5 61% ±7 93% ±11 Plasmid (general) 2 µg 100 µl I/II/2b
Endothelial, basic T-005 4e5 59% ±7 92% ±11 Plasmid (general) 2 µg 100 µl I/II/2b

Citations

Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Clarke PA, Dickson JH, Harris JC, Grabowska A, Watson SA. 
In:
Cancer Res (2006) 66(7): 3504-12 
Categories:
Primary Cells and Media 
Authors:
Chi JT, Chang HY, Haraldsen G, Jahnsen FL, Troyanskaya OG, Chang DS, Wang Z, Rockson SG, van de Rijn M, Botstein D, Brown PO. 
In:
Proc Natl Acad Sci USA (2003) 100 (19): 10623-8