22Rv1

prostate carcinoma

Cell Type:
Prostate Epith.
Tissue Origin:
prostate
Species:
human
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
V A-020 2e6 50% 80% Plasmid (general) 2 µg 100 µl I/II/2b
V X-001 2e6 60% 70% Plasmid (general) 2 µg 100 µl I/II/2b
L X-001 2e6 50% 80% Plasmid (general) 2 µg 100 µl I/II/2b

Citations

Categories:
Transfection 
Authors:
Sándor Spisák, Kate Lawrenson, Yanfang Fu, István Csabai, Rebecca T Cottman, Ji-Heui Seo, Christopher Haiman, Ying Han, Romina Lenci, Qiyuan Li, Viktória Tisza, Zoltán Szállási, Zachery T Herbert, Matthew Chabot, Mark Pomerantz, Norbert Solymosi, The GAME-ON/ELLIPSE Consortium, Simon A Gayther, J Keith Joung, Matthew L Freedman 
In:
Nat Med (2015) 21(11): 1357-63 
Categories:
Live Cell Imaging 
Authors:
Orellana-Serradell O1, Herrera D1, Castellon EA1, Contreras HR1. 
In:
Asian J Androl () 3: 294-299