cells

Human Chondrocyte Cell Line established by transfecting primary cultures (day 5) of costal cartilage from a 15-year-old female with a retroviral vector expressing simian virus SV40 large T antigen by Mary B. Goldring et al. (1994)

Cell Type:

Cartilage

Tissue Origin:

cartilage

Species:

human

Research Area:

Dermatology/Tissue Engineering

Regenerative medicine

Cell Characteristics:

Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Protocol	Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
	V	X-001	1e6	90% ±2	80% ±1	Plasmid (general)	2 µg	100 µl	I/II/2b

T/C-28 a2

Transfection Information