L-363
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established from the peripheral blood of a 36-year-old woman with plasma cell leukemia (IgG) in 1977
Cell Type:
B cell
Tissue Origin:
blood
Species:
human
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
Filter:
Any Substrate
Any Platform
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
SF
DN-100
1e6
58%
65%
Plasmid (general)
400 ng
4D X-Unit
SF
EH-100
1e6
51%
59%
Plasmid (general)
400 ng
4D X-Unit
SF
EO-100
1e6
47%
70%
Plasmid (general)
400 ng
4D X-Unit
SF
EN-138
1e6
54%
53%
Plasmid (general)
400 ng
4D X-Unit