293H

The 293-H strain is a variant of the 293 cell line that demonstrates better adherence in monolayer culture and ease of use for plaque assays and other anchorage dependent applications

Cell Type:
Kidney Epith.
Tissue Origin:
kidney
Species:
human
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent

Recommended Media

Pro293 Chemically Defined, Serum-free Media were optimized to support high-density growth and recombinant protein production in 293 human kidney cells. They are chemically defined to ease regulatory compliance and downstream protein purification. They contain very low levels of recombinant human insulin, and are free of animal-origin components.

Pro293s Medium - for 293 cells growing in suspension culture
Pro293a Medium - for 293 cells growing in adherent culture


Storage = 2°C to 8°C

12-765 - Pro293s Medium (without L-glutamine or phenol red, with 0.1% Pluronic F-68)

12-764 - Pro293a Medium (without L-glutamine or phenol red, with 0.1% Pluronic F-68)
BE02-025Q - Pro293a Medium (without L-glutamine, galaktose or phenol red, with 0.1% Pluronic F-68)

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
V Q-001 1e6 70% good Plasmid (general) 100 µl I/II/2b

Citations

Categories:
 
Authors:
Efren Garcia-Maldonado , Andrew D Huber , Sergio C Chai , Stanley Nithianantham , Yongtao Li , Jing Wu , Shyaron Poudel , Darcie J Miller , Jayaraman Seetharaman , Taosheng Chen  
In:
Nature (2024) 15: 4054 
Categories:
Transfection 
Authors:
Tanner Rathbone , Ilayda Ates , Lawrence Fernando , Ethan Addlestone , Ciaran M Lee , Vincent P Richards , Renee N Cottle de  
In:
CRISPR J (2022) :