NRK-49F

Spontaneously immortalized rat kidney fibroblast cell line

Cell Type:
Fibroblast
Tissue Origin:
kidney
Species:
rat
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
T T-030 54% Plasmid (general) 2 µg 100 µl I/II/2b
T X-005 46% Plasmid (general) 2 µg 100 µl I/II/2b
T T-020 43% Plasmid (general) 2 µg 100 µl I/II/2b