Spontaneously immortalized rat kidney fibroblast cell line
Dulbecco's Modified Eagle Medium (DMEM) was developed in 1969 and is a modification of Basal Medium Eagle (BME) that differs from BME and MEM by the following characteristics:
It is used in a wide range of mammalian cell culture applications. The high glucose version is well suited to high density suspension culture. The low glucose formula is used for adherent dependent cells.Storage = 2ºC to 8ºCLiquid:12-614 = 4.5 g/L glucose, without L-glutamine12-604 = 4.5 g/L glucose, with L-glutamineBE12-604/U1 = 4.5 g/L glucose, with Ultraglutamine I12-914 = 4.5 g/L glucose, without L-glutamine (hybridoma screened)12-917 = 4.5 g/L glucose, without L-glutamine or phenol red12-709 = 4.5 g/L glucose and 25 mM HEPES, without L-glutamine12-733= 4.5 g/L glucose, without L-glutamine or sodium pyruvate12-741 = 4.5 g/L glucose, with L-glutamine, without sodium pyruvate12-707 = 1.0 g/L glucose, without L-glutamine12-708 = 1.0 g/L glucose and 25 mM HEPES, without L-glutaminePowder:15-614 = 4.5 g/L glucose, without L-glutamine or sodium pyruvate15-604 = 4.5 g/L glucose, with L-glutamine or sodium pyruvate
PC-1™ is a low-protein, serum-free medium intended for the culture of primary cells and anchorage-dependent cell lines. PC-1 is formulated in a specially modified DMEM/F12 base and contains a complete HEPES buffering system with known amounts of insulin, transferrin, fatty acids, and proprietary proteins assembled under strict quality control procedures. PC-1™ is intended for a variety of research and industrial applications and is formulated using defined components for optimal cell growth, while maintaining the lowest possible protein content.PC-1™ does not contain L-glutamine.PC-1™ Liquid Base Medium is to be stored at 2°C-8°C.The Supplement should be stored at -20°C.When these two components are combined, the resulting PC-1™ Complete Medium is stable for 45 days at 2°C-8°C.Once thawed, the appropriate volume of one vial of PC-1™ Supplement must be combined with the companion volume of PC-1™ Liquid Base Medium. Partial reconstitution or repeated freezing and thawing of the PC-1™ Supplement is not advised.
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