cells

Mammary gland epithelial

Cell Type:

Mammary Epith.

Tissue Origin:

breast

Species:

human

Research Area:

Cancer Research/Cell Biology

Cell Characteristics:

Adherent

Recommended Media

Mammary Epithelial Cell Growth Medium

Serum-free media developed specifically for growth of mammary epithelial cells.

Available in phenol red-free (basal media = CC3153) and sodium bicarbonate-free (basal media = CC-3152) versions.

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Protocol	Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
	V	T-030	1e6	80%	80%	Plasmid (general)	2 µg	100 µl	I/II/2b
	V	T-020	1e6	70%	80%	Plasmid (general)	2 µg	100 µl	I/II/2b

Citations

An evolutionarily conserved DNA architecture determines target specificity of the TWIST family bHLH transcription factors

Categories:

Authors:

Chang AT, Liu Y, Ayyanathan K, Benner C, Jiang Y, Prokop JW, Paz H, Wang D, Li HR, Fu XD, Rauscher FJ 3rd, Yang J.

In:

Genes Dev (2015) 29(6): 603-616

miR-205 acts as a tumor radiosensitizer by targeting ZEB1 and Ubc13

Categories:

Authors:

Zhang P1, Wang L, Rodriguez-Aguayo C, Yuan Y, Debeb BG, Chen D, Sun Y, You MJ, Liu Y, Dean DC, Woodward WA, Liang H, Yang X, Lopez-Berestein G, Sood AK, Hu Y, Ang KK, Chen J, Ma L

In:

Nat Commun. (2014) 5(5): 5671

HMLE

Recommended Media

Transfection Information

Citations