MCF7 tet
Export
mammary gland adenocarcinoma, tet on off system
Cell Type:
Mammary Epith.
Tissue Origin:
breast
Species:
human
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
V
T-016
1e6
56%
74%
Plasmid (general)
2 µg
100 µl
I/II/2b
V
G-016
1e6
61%
88%
Plasmid (general)
2 µg
100 µl
I/II/2b
V
E-014
1e6
61%
87%
Plasmid (general)
2 µg
100 µl
I/II/2b
V
P-020
1e6
66%
80%
Plasmid (general)
2 µg
100 µl
I/II/2b
Citations (7)
Selective inhibition of histone deacetylase 2 silences progesterone receptor-mediated signaling
Categories:
Transfection
Authors:
Bicaku E, Marchion DC, Schmitt ML, Münster PN
In:
Cancer Res (2008) 68(5): 1513-9
Breast cancer antiestrogen resistance-3 expression regulates breast cancer cell migration through promotion of p130Cas membrane localization and membrane ruffling
Categories:
Transfection
Authors:
Schrecengost RS, Riggins RB, Thomas KS, Guerrero MS, Bouton AH
In:
Cancer Res (2007) 67(13): 6174-82
Regulation of the Raf-MEK-ERK pathway by protein phosphatase 5
Categories:
Transfection
Authors:
von Kriegsheim A, Pitt A, Grindlay GJ, Kolch W, Dhillon AS
In:
Nat Cell Biol (2006) 8(9): 1011-6
Tumor invasion in the absence of epithelial-mesenchymal transition: podoplanin-mediated remodeling of the actin cytoskeleton
Categories:
Transfection
Authors:
Wicki A, Lehembre F, Wick N, Hantusch B, Kerjaschki D, Christofori G
In:
Cancer Cell (2006) 9(4): 261-72
Reduction of Raf-1 kinase inhibitor protein expression correlates with breast cancer metastasis
Categories:
Transfection
Authors:
Hagan S, Al-Mulla F, Mallon E, Oien K, Ferrier R, Gusterson B, Garcia JJ and Kolch W
In:
Clin Cancer Res (2005) 11(20): 7392-7397
IQGAP1 Is a Scaffold for Mitogen-Activated Protein Kinase Signaling
Categories:
Transfection
Authors:
Roy M, Li Z and Sacks DB
In:
Mol Cell Biol (2005) 25(18): 7940-7952
The BRG1- and hBRM-Associated Factor BAF57 Induces Apoptosis by Stimulating Expression of the Cylindromatosis Tumor Suppressor Gene
Categories:
Transfection
Authors:
Wang L, Baiocchi RA, Pal S, Mosialos G, Caligiuri M and Sif S
In:
Mol Cell Biol (2005) 25(18): 7953-7965
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