cells

Cells derived from the wall of the blastocyst. After implantation trophoblast divides into two layers, the chorion and the placenta.

Cell Type:

Embryonic/Extraembryonic Epith.

Tissue Origin:

embryo

Species:

human

Research Area:

Cancer Research/Cell Biology

Cell Characteristics:

Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Protocol	Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
	NHDF	G-016	6e5	95%	75%	Plasmid (general)	3 µg	100 µl	I/II/2b

Citations

Methods for siRNA-mediated reduction of mRNA and protein expression in human placental explants, isolated primary cells and cell lines.

Categories:

Transfection

Authors:

Forbes K, Desforges M, Garside R, Aplin JD, Westwood M.

In:

Placenta (2009) 30 (2): 124-129

In vitro restoration of functional SMN protein in human trophoblast cells affected by spinal muscular atrophy by small fragment homologous replacement

Categories:

Transfection

Authors:

Sangiuolo F, Filareto A, Spitalieri P, Scaldaferri ML, Mango R, Bruscia E, Citro G, Brunetti E, De Felici M, Novelli G

In:

Hum Gene Ther (2005) 16(7): 869-80

Trophoblast, human

Transfection Information

Citations