cells

A murine pre-B cell line transformed using Abelson leukemia virus

Cell Type:

B cell

Tissue Origin:

blood

Species:

mouse

Research Area:

Cancer Research/Cell Biology

Immunotherapy / Hematology

Cell Characteristics:

Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
V	T-020	2e6	54%	81%	Plasmid (general)	2.5 µg	100 µl	I/II/2b
V	T-023	2e6	50%	84%	Plasmid (general)	2.5 µg	100 µl	I/II/2b
V	U-015	2e6	55%	78%	Plasmid (general)	2.5 µg	100 µl	I/II/2b
V	T-020	2e6	97%	84%	Plasmid (general)	2.5 µg	100 µl	I/II/2b
V	U-015	2e6	98%	72%	Plasmid (general)	2.5 µg	100 µl	I/II/2b
V	T-023	2e6	97%	62%	Plasmid (general)	2.5 µg	100 µl	I/II/2b

300.19

Transfection Information