cells

human osteosarcoma

Cell Type:

Bone

Tissue Origin:

bone

Species:

human

Research Area:

Cancer Research/Cell Biology

Cell Characteristics:

Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Protocol	Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
	T	T-027	1e6	70%	70%	Plasmid (general)	2 µg	100 µl	I/II/2b

Citations

Development of a single-chain, quasi-dimeric zinc-finger nuclease for the selective degradation of mutated human mitochondrial DNA

Categories:

Transfection

Authors:

Minczuk M, Papworth MA, Miller JC, Murphy MP, Klug A

In:

Nucleic Acids Res (2008) 36(12): 3926-38

Sequence-specific modification of mitochondrial DNA using a chimeric zinc finger methylase

Categories:

Transfection

Authors:

Minczuk M, Papworth MA, Kolasinska P, Murphy MP, Klug A

In:

Proc Natl Acad Sci USA (2006) 103(52): 19689-94

143B

Transfection Information

Citations