143B
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Human osteosarcoma cell line derived from bone tumor
Cell Type:
Bone
Tissue Origin:
bone
Species:
human
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
Filter:
Any Substrate
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
T
T-027
1e6
70%
70%
Plasmid (general)
2 µg
100 µl
I/II/2b
SF
FP-133
2e5
CRISPR/Cas
20 µl
4D X-Unit
Citations
Mitochondrial redox adaptations enable alternative aspartate synthesis in SDH-deficient cells
Categories:
Transfection
Authors:
Madeleine L Hart, Evan Quon, Anna-Lena BG Vigil, Ian A Engstrom, Oliver J Newsom, Kristian Davidsen, Pia Hoellerbauer, Samantha M Carlisle, Lucas B Sullivan
In:
eLife (2023) :
Development of a single-chain, quasi-dimeric zinc-finger nuclease for the selective degradation of mutated human mitochondrial DNA
Categories:
Transfection
Authors:
Minczuk M, Papworth MA, Miller JC, Murphy MP, Klug A
In:
Nucleic Acids Res (2008) 36(12): 3926-38
Sequence-specific modification of mitochondrial DNA using a chimeric zinc finger methylase
Categories:
Transfection
Authors:
Minczuk M, Papworth MA, Kolasinska P, Murphy MP, Klug A
In:
Proc Natl Acad Sci USA (2006) 103(52): 19689-94