cells

A mesenchymal fibroblast-like cell line of embryonic origin that can be induced to evolve into osteogenic or myofibroblast cell lineages

Cell Type:

Mesenchyme

Tissue Origin:

embryo

Species:

mouse

Research Area:

Cancer Research/Cell Biology

Dermatology/Tissue Engineering

Cell Characteristics:

Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
SE	96-CM-137	2e5			Plasmid (general)	200 ng	20 µl	96-well
V	A-020	1e6	90%	90%	Plasmid (general)	2 µg	100 µl	I/II/2b
V	T-020	1e6	85%	80%	Plasmid (general)	2 µg	100 µl	I/II/2b

Citations

Regulation of Myogenic Differentiation by Androgens: Cross-Talk between Androgen Receptor/beta-Catenin and Follistatin/TGF-beta Signaling Pathways

Categories:

Transfection

Authors:

Singh R, Bhasin S, Braga M, Artaza JN, Pervin S, Taylor WE, Krishnan V, Sinha SK, Rajavashisth TB, Jasuja R

In:

Endocrinology (2008) epub: online

Conservation analysis predicts in vivo occupancy of glucocorticoid receptor-binding sequences at glucocorticoid-induced genes

Categories:

Transfection

Authors:

So AY, Cooper SB, Feldman BJ, Manuchehri M, Yamamoto KR

In:

Proc Natl Acad Sci USA (2008) 105(15): 5745-9

C3H10T1/2

Transfection Information

Citations