C3H10T1/2

A mesenchymal fibroblast-like cell line of embryonic origin that can be induced to evolve into osteogenic or myofibroblast cell lineages
Cell Type:
Mesenchyme
Tissue Origin:
embryo
Species:
mouse
Research Area:
Cancer Research/Cell Biology
Dermatology/Tissue Engineering
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SE 96-CM-137 2e5 Plasmid (general) 200 ng 20 µl Shuttle
V A-020 1e6 90% 90% Plasmid (general) 2 µg 100 µl I/II/2b
V T-020 1e6 85% 80% Plasmid (general) 2 µg 100 µl I/II/2b

Citations

Categories:
Transfection 
Authors:
Singh R, Bhasin S, Braga M, Artaza JN, Pervin S, Taylor WE, Krishnan V, Sinha SK, Rajavashisth TB, Jasuja R 
In:
Endocrinology (2008) epub: online 
Categories:
Transfection 
Authors:
So AY, Cooper SB, Feldman BJ, Manuchehri M, Yamamoto KR 
In:
Proc Natl Acad Sci USA (2008) 105(15): 5745-9