cells

Mouse peripheral blood lymphocyte

Cell Type:

B cell

Tissue Origin:

blood

Species:

mouse

Research Area:

Immunotherapy / Hematology

Cell Characteristics:

Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
P4	DI-100-AA	6e5	57-59%	40-85%	Plasmid (general)	0.4 µg	20 µl	384-well
P4	DI-100	6e5	51-61%	33-49%	Plasmid (general)	0.4 µg	20 µl	4D X-Unit
P4	DI-100	6e5	55% ±24	87% ±18	Plasmid (general)	0.4 µg	20 µl	4D X-Unit
B cell, mouse	Z-001	3e6	49% ±4	27% ±13	Plasmid (general)	2 µg	100 µl	I/II/2b
B cell, mouse	Z-001	3e6	59% ±6	47% ±25	Plasmid (general)	2 µg	100 µl	I/II/2b
P4	96-DI-100	6e5	56% ±5	41% ±8	Plasmid (general)	0.4 µg	20 µl	96-well
P4	96-DI-100	6e5	55% ±24	87% ±18	Plasmid (general)	0.4 µg	20 µl	96-well

Citations

The rap GTPases regulate B cell morphology, immune-synapse formation, and signaling by particulate B cell receptor ligands

Categories:

Transfection

Authors:

Lin KB, Freeman SA, Zabetian S, Brugger H, Weber M, Lei V, Dang-Lawson M, Tse KW, Santamaria R, Batista FD, Gold MR

In:

Immunity (2008) 28(1): 75-87

B-cell, mouse, stimulated

Transfection Information

Citations