human caucasian ovary adenocarcinoma

Cell Type:
Ovary Epith.
Tissue Origin:
Research Area:
Cancer Research/Cell Biology
Dermatology/Tissue Engineering
Cell Characteristics:

Recommended Media

McCoy's 5A Medium was developed in 1956 to be formulated for the nutritional requirements of Walker 256 carcinoma. Its unique components include asparagine, 3X normal glucose, amino acids (all Lisomers) and increased folic acid. It is a general purpose medium for primary and established cell lines.

12-168 With L-glutamine and 25 mM HEPES 
12-688 With L-glutamine

Storage = 2ºC to 8ºC

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SF FE-132 2e5 86% ±4 66% ±1 Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SF FE-132 1e6 84% ±1 68% ±17 Plasmid (general) 2 µg 100 µl 4D X-Unit
V V-005 1e6 89% ±6 53% ±3 Plasmid (general) 2 µg 100 µl I/II/2b


Cao Q, Lu X, Feng YJ 
Cell Res (2006) 16(7): 671-7