J-Lat 6.2

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Jurkat-derived T-cell clones infected with a single copy of a full-length HIV-1 provirus engineered to express green fluorescent protein (GFP) in lieu of Nef

Cell Type:
T Cell
Tissue Origin:
blood
Species:
human
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
R O-028 5e6 95% siRNA 5 µg 100 µl I/II/2b

Citations

Sustained induction of NF-kappaB is required for efficient expression of latent human immunodeficiency virus type 1
Categories:
Transfection 
Authors:
Williams SA, Kwon H, Chen LF, Greene WC 
In:
J Virol (2007) 81(11): 6043-56 

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