HFF-1
Export
pooled normal human foreskin
Cell Type:
Fibroblast
Tissue Origin:
dermal
Species:
human
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
Filter:
Any Substrate
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
SE
DS-137, FF-150, FF-130, DS-150, FF-138
2e5
90+ %
good
Plasmid (general)
0.4 µg
20 µl
96-well
Citations
Reprogramming triggers endogenous L1 and Alu retrotransposition in human induced pluripotent stem cells.
Categories:
Transfection
Authors:
Klawitter S, Fuchs NV, Upton KR, Muñoz-Lopez M, Shukla R, Wang J, Garcia-Cañadas M, Lopez-Ruiz C, Gerhardt DJ, Sebe A, Grabundzija I, Merkert S, Gerdes P Pulgarin JA, Bock A, Held U, Witthuhn A, Haase A, Sarkadi B, Löwer J, Wolvetang EJ, Martin U, Ivics Z, Izsvák Z, Garcia-Perez JL, Faulkner GJ, Schumann GG
In:
Nat Commun. (2016) 7: 10286
Dual-color superresolution imaging of genetically expressed probes within individual adhesion complexes
Categories:
Transfection
Authors:
Shroff H, Galbraith CG, Galbraith JA, White H, Gillette J, Olenych S, Davidson MW, Betzig E
In:
Proc Natl Acad Sci USA (2007) 104(51): 20308-13