cells

A three-dimensional model of the human corneal epithelium. The model consists of human corneal epithelial cells cultured on permeable membrane supports. Cells are immortalized by infection with an amphotropic recombinant retrovirus containing HPV-16 E6/E7 genes.

Cell Type:

Eye Epith.

Tissue Origin:

eye

Species:

human

Research Area:

Cancer Research/Cell Biology

Cell Characteristics:

Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Protocol	Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
		U-020	5e5	50%		Plasmid (general)	1 µg	100 µl	I/II/2b

Citations

Enzymes responsible for synthesis of corneal keratan sulfate glycosaminoglycans

Categories:

Transfection

Authors:

Kitayama K, Hayashida Y, Nishida K, Akama TO

In:

J Biol Chem (2007) 282(41): 30085-96

Urokinase receptor cleavage: a crucial step in fibroblast-to-myofibroblast differentiation

Categories:

Transfection

Authors:

Bernstein AM, Twining SS, Warejcka DJ, Tall E, Masur SK

In:

Mol Biol Cell (2007) 18(7): 2716-27

Epithelial model, cornea, human, immort.

Transfection Information

Citations