cells

EBV-transformed B cells

Cell Type:

B cell

Tissue Origin:

blood

Species:

human

Research Area:

Cancer Research/Cell Biology

Immunotherapy / Hematology

Cell Characteristics:

Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Protocol	Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
	V	O-017		10-30%	high	Plasmid (general)	µg	100 µl	I/II/2b
	V	T-020		25-38%	low viability	Plasmid (general)	µg	100 µl	I/II/2b

Citations (18)

Adenine base editing efficiently restores the function of Fanconi anemia hematopoietic stem and progenitor cells

Categories:

Transfection

Authors:

Siegner SM, Ugalde L, Clemens A, Garcia-Garcia L, Bueren JA, Rio P, Karasu ME, Corn JE.

In:

Nat Commun. (2022) 13(1): 6900

Highly efficient induced pluripotent stem cell reprogramming of cryopreserved lymphoblastoid cell lines

Categories:

Transfection

Authors:

Satish Kumar , Joanne E Curran , Erika C Espinosa , David C Glahn , John Blangero

In:

J Bio Med Opt (2020) 7(1): 124

CRISPR/Cas9-Mediated Genome Editing in Epstein-Barr Virus-Transformed Lymphoblastoid B-Cell Lines.

Categories:

Transfection, Cell Lines and Primary Cancer Cells

Authors:

Jiang S, Wang LW, Walsh MJ, Trudeau SJ, Gerdt C, Zhao B, Gewurz BE.

In:

Curr Biol (2018) 121: 31

Current protocols of molecular biology: CRISPR/Cas9-Mediated Genome Editing in Epstein-Barr Virus-Transformed Lymphoblastoid B-Cell Lines.

Categories:

Transfection, Cell Lines and Primary Cancer Cells

Authors:

Jiang S, Wang LW, Walsh MJ, Trudeau SJ, Gerdt C, Zhao B, Gewurz BE.

In:

(2018) 121: 31

Reprogramming LCLs to iPSCs Results in Recovery of Donor-Specific Gene Expression Signature.

Categories:

Transfection

Authors:

Thomas SM, Kagan C, Pavlovic BJ, Burnett J, Patterson K, Pritchard JK, Gilad Y

In:

PLoS Genet (2015) 11(5): e1005216

Human lymphoblastoid B-cell lines reprogrammed to EBV-free induced pluripotent stem cells

Categories:

Transfection

Authors:

Deepika Rajesh, Sarah J. Dickerson, Junying Yu, Matthew E. Brown, James A. Thomson, and Nicholas J. Seay

In:

Blood (2011) 118 (7): 1797-1800

Gene knockdown studies revealed CCDC50 as a candidate gene in mantle cell lymphoma and chronic lymphocytic leukemia

Categories:

Transfection

Authors:

Farfsing A, Engel F, Seiffert M, Hartmann E, Ott G, Rosenwald A, Stilgenbauer S, Döhner H, Boutros M, Lichter P, Pscherer A.

In:

Leukemia (2009) 23(11): 2018-26

Molecular Basis of Epstein-Barr Virus Latent Membrane Protein 1 (LMP1) Cytotoxicity in EBV Latency III B-cells: LMP1 induces type II Ligand independent Autoactivation of CD95/Fas with Caspase-8 mediated Apoptosis

Categories:

Transfection

Authors:

Le Clorennec C, Ouk TS, Youlyouz-Marfak I, Panteix S, Martin CC, Rastelli J, Adriaenssens E, Zimber-Strobl U, Coll J, Feuillard J, Jayat-Vignoles C

In:

J Virol (2008) 82(13): 6721-33

Epstein-Barr virus latent membrane protein 2A is a B-cell receptor mimic and essential for B-cell survival

Categories:

Transfection

Authors:

Mancao C, Hammerschmidt W

In:

Blood (2007) 110(10): 3715-21

X-box-binding protein 1 (XBP-1) activates lytic Epstein-Barr virus gene expression in combination with Protein Kinase D (PKD)

Categories:

Transfection

Authors:

Bhende PM, Dickerson SJ, Sun X, Feng WH, Kenney SC

In:

J Virol (2007) 81(14): 7363-70

c-Rel plays a key role in deficient activation of B cells from a non-X-linked hyper IgM patient

Categories:

Transfection

Authors:

Lu KT, Sinquett FL, Dryer RL, Song C, Covey LR

In:

Blood (2006) 108(12): 3769-76

Fine Mapping of Genetic Susceptibility to Polycystic Ovary Syndrome on Chromosome 19p13.2 and Tests for Regulatory Activity

Categories:

Transfection

Authors:

Stewart DR, Dombroski B, Urbanek M, Ankener W, Ewens KG, Wood JR, Legro RS, Strauss JF 3rd, Dunaif A, Spielman RS

In:

J Clin Endocrinol Metab (2006) 91(10): 4112-4117

A Role for Intercellular Antigen Transfer in the Recognition of EBV-Transformed B Cell Lines by EBV Nuclear Antigen-Specific CD4+ T Cells

Categories:

Transfection

Authors:

Taylor GS, Long HM, Haigh TA, Larsen M, Brooks J, Rickinson AB

In:

J Immunol (2006) 177(6): 3746-56

Detection of functional single-nucleotide polymorphisms that affect apoptosis

Categories:

Transfection

Authors:

Harris SL, Gil G, Robins H, Hu W, Hirshfield K, Bond E, Bond G and Levine AJ

In:

Proc Natl Acad Sci USA (2005) 102(45): 16297-16302

Epstein-barr virus lytic infection is required for efficient production of the angiogenesis factor vascular endothelial growth factor in lymphoblastoid cell lines

Categories:

Transfection

Authors:

Hong GK, Kumar P, Wang L, Damania B, Gulley ML, Delecluse HJ, Polverini PJ and Kenney SC

In:

J Virol (2005) 79(22): 13984-13992

Epstein-Barr virus lytic infection contributes to lymphoproliferative disease in a SCID mouse model

Categories:

Transfection

Authors:

Hong GK, Gulley ML, Feng WH, Delecluse HJ, Holley-Guthrie E and Kenney SC

In:

J Virol (2005) 79(22): 13993-14003

Determination of Cellularly Processed HLA-A2402-Restricted Novel CTL Epitopes Derived from Two Cancer Germ Line Genes, MAGE-A4 and SAGE

Categories:

Transfection

Authors:

Miyahara Y, Naota H, Wang L, Hiasa A, Goto M, Watanabe M, Kitano S, Okumura S, Takemitsu T, Yuta A, Majima Y, Lemonnier FA, Boon T and Shiku H

In:

Clin Cancer Res (2005) 11(15): 5581-5589

The BRRF1 early gene of Epstein-Barr virus encodes a transcription factor that enhances induction of lytic infection by BRLF1

Categories:

Transfection

Authors:

Hong GK, Delecluse HJ, Gruffat H, Morrison TE, Feng WH, Sergeant A and Kenney SC

In:

J Virol (2004) 78(10): 4983-4992

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