PC-3

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human prostate adenocarcinoma (established from bone marrow metastasis)

Cell Type:
Prostate Epith.
Tissue Origin:
prostate
Species:
human
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SF DS-137 1e5 84% ±1 81% ±1 Plasmid (general) 400 ng 20 µl 4D X-Unit
SF DS-137 5e5 82% ±1 77% ±12 Plasmid (general) 2 µg 100 µl 4D X-Unit
V T-013 1e6 81% ±11 66% Plasmid (general) 2 µg 100 µl I/II/2b
V T-013 1e6 88% ±8 59% Plasmid (general) 2 µg 100 µl I/II/2b

Citations (11)

Vascular endothelial growth factor receptor-1 contributes to resistance to anti-epidermal growth factor receptor drugs in human cancer cells
Categories:
Transfection 
Authors:
Bianco R, Rosa R, Damiano V, Daniele G, Gelardi T, Garofalo S, Tarallo V, De Falco S, Melisi D, Benelli R, Albini A, Ryan A, Ciardiello F, Tortora G 
In:
Clin Cancer Res (2008) 14(16): 5069-80 
Multiple Cellular Mechanisms Related to Cyclin A1 in Prostate Cancer Invasion and Metastasis
Categories:
Transfection 
Authors:
Wegiel B, Bjartell A, Tuomela J, Dizeyi N, Tinzl M, Helczynski L, Nilsson E, Otterbein LE, Harkonen P, Persson JL 
In:
J Natl Cancer Inst (2008) 100(14): 1022-36 
Promotion of neurite outgrowth by fibroblast growth factor receptor 1 overexpression and lysosomal inhibition of receptor degradation in pheochromocytoma cells and adult sensory neurons
Categories:
Transfection 
Authors:
Hausott B, Schlick B, Vallant N, Dorn R, Klimaschewski L 
In:
Neuroscience (2008) 153(2): 461-73 
Hypoxia regulates choline kinase expression through hypoxia-inducible factor-1 alpha signaling in a human prostate cancer model
Categories:
Transfection 
Authors:
Glunde K, Shah T, Winnard PT Jr, Raman V, Takagi T, Vesuna F, Artemov D, Bhujwalla ZM 
In:
Cancer Res (2008) 68(1): 172-80 
AP-2 regulates the transcription of estrogen receptor (ER)-beta by acting through a methylation hotspot of the 0N promoter in prostate cancer cells
Categories:
Transfection 
Authors:
Zhang X, Leung YK, Ho SM 
In:
Oncogene (2007) 26(52): 7346-54 
Inhibitory role of RhoA on senescence-like growth arrest by a mechanism involving modulation of phosphatase activity
Categories:
Transfection 
Authors:
Park C, Lee I, Jang JH, Kang WK 
In:
FEBS Lett (2007) 581(20): 3800-4 
Prostate cell differentiation status determines transient receptor potential melastatin member 8 channel subcellular localization and function
Categories:
Transfection 
Authors:
Bidaux G, Flourakis M, Thebault S, Zholos A, Beck B, Gkika D, Roudbaraki M, Bonnal JL, Mauroy B, Shuba Y, Skryma R, Prevarskaya N 
In:
J Clin Invest (2007) 117(6): 1647-1657 
Luteolin inhibits insulin-like growth factor 1 receptor signaling in prostate cancer cells
Categories:
Transfection 
Authors:
Fang J, Zhou Q, Shi XL, Jiang BH 
In:
Carcinogenesis (2007) 28(3): 713-23 
S100A4 accelerates tumorigenesis and invasion of human prostate cancer through the transcriptional regulation of matrix metalloproteinase 9
Categories:
Transfection 
Authors:
Saleem M, Kweon MH, Johnson JJ, Adhami VM, Elcheva I, Khan N, Bin Hafeez B, Bhat KM, Sarfaraz S, Reagan-Shaw S, Spiegelman VS, Setaluri V, Mukhtar H 
In:
Proc Natl Acad Sci USA (2006) 103(40): 14825-30 
A role for cyclin A1 in mediating the autocrine expression of vascular endothelial growth factor in prostate cancer
Categories:
Transfection 
Authors:
Wegiel B, Bjartell A, Ekberg J, Gadaleanu V, Brunhoff C and Persson JL 
In:
Oncogene (2005) 24(42): 6385-6393 
The transcription factor ZEB1 promotes an aggressive phenotype in prostate cancer cell lines
Categories:
Live Cell Imaging 
Authors:
Orellana-Serradell O1, Herrera D1, Castellon EA1, Contreras HR1. 
In:
Asian J Androl () 3: 294-299 
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