cells

mouse-rat T cell hybridoma

Cell Type:

T Cell

Tissue Origin:

blood

Species:

cell hybrid

Research Area:

Immunotherapy / Hematology

Cell Characteristics:

Suspension

Recommended Media

Iscove's Modified Dulbecco's Medium

Iscove's Modified Dulbecco's Medium (IMDM) is a modified DME containing high glucose (4,500 mg/L), sodium pyruvate, additional amino acids, HEPES buffer (in liquid form), selenium and other components. IMDM is suitable for fast growing cells and can support the growth of a wide variety of mammalian cells.

Storage = 2ºC to 8ºC

12-722 with L-glutamine
12-726 without L-glutamine
12-915 with L-glutamine and screened to support hybridoma growth

UltraDOMA-Protein Free

UltraDOMA-PF Protein-free Hydridoma Media are formulations designed for use with hybridoma cell lines of murine, human, and chimeric origin. UltraDOMA-PF Media are completely defined media and do not contain peptides or tissue extracts.
The use of UltraDOMA-PF Media significantly simplifies downstream processing since all proteins present in a given cell culture supernatant are produced by the cells.

L-glutamine and HEPES buffer are included in the formulation.

Storage = 2ºC to 8ºC

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Protocol	Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
	V	T-020	1e6	38%	75%	Plasmid (general)	1 µg	100 µl	I/II/2b
	V	T-030	1e6	56%	74%	Plasmid (general)	1 µg	100 µl	I/II/2b

SAM-19

Recommended Media

Transfection Information