PC-12

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rat adrenal pheochromocytoma

Cell Type:
Adrenal Cells
Tissue Origin:
adrenal
Species:
rat
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SF EI-100 2e6 82% ±1 54% ±6 Plasmid (general) 2 µg 100 µl 4D X-Unit
SF EI-100 4e5 83% ±2 57% ±4 Plasmid (general) 400 ng 20 µl 4D X-Unit
V U-029 2e6 92.3% ±3 80.8% ±5 Plasmid (general) 2 µg 100 µl I/II/2b

Citations (24)

The stargazin-related protein gamma 7 interacts with the mRNA-binding protein heterogeneous nuclear ribonucleoprotein A2 and regulates the stability of specific mRNAs, including CaV2.2
Categories:
Transfection 
Authors:
Ferron L, Davies A, Page KM, Cox DJ, Leroy J, Waithe D, Butcher AJ, Sellaturay P, Bolsover S, Pratt WS, Moss FJ, Dolphin AC 
In:
J Neurosci (2008) 28(42): 10604-17 
A cAMP-dependent, protein kinase A-independent signaling pathway mediating neuritogenesis through Egr1 in PC12 cells
Categories:
Transfection 
Authors:
Ravni A, Vaudry D, Gerdin MJ, Eiden MV, Falluel-Morel A, Gonzalez B, Vaudry H, Eiden LE 
In:
Mol Pharmacol (2008) 73(6): 1688-708 
Processing and nuclear localization of CRMP2 during brain development induce neurite outgrowth inhibition
Categories:
Transfection 
Authors:
Rogemond V, Auger C, Giraudon P, Becchi M, Auvergnon N, Belin MF, Honnorat J, Moradi-Améli M 
In:
J Biol Chem (2008) 283(21): 14751-61 
Promotion of neurite outgrowth by fibroblast growth factor receptor 1 overexpression and lysosomal inhibition of receptor degradation in pheochromocytoma cells and adult sensory neurons
Categories:
Transfection 
Authors:
Hausott B, Schlick B, Vallant N, Dorn R, Klimaschewski L 
In:
Neuroscience (2008) 153(2): 461-73 
Synaptic function for the Nogo-66 receptor NgR1: regulation of dendritic spine morphology and activity-dependent synaptic strength
Categories:
Transfection 
Authors:
Lee H, Raiker SJ, Venkatesh K, Geary R, Robak LA, Zhang Y, Yeh HH, Shrager P, Giger RJ 
In:
J Neurosci (2008) 28(11): 2753-65 
Snapin, a new regulator of receptor signaling, augments alpha 1A-adrenoceptor-operated calcium influx through TRPC6
Categories:
Transfection 
Authors:
Suzuki F, Morishima S, Tanaka T, Muramatsu I 
In:
J Biol Chem (2007) 282(40): 29563-73 
Glycogen synthase kinase-3beta induces neuronal cell death via direct phosphorylation of mixed-lineage K3
Categories:
Transfection 
Authors:
Mishra R, Barthwal MK, Sondarva G, Rana B, Wong L, Chatterjee M, Woodgett JR, Rana A 
In:
J Biol Chem (2007) 282(42): 30393-405 
Antiepileptic drugs affect neuronal androgen signaling via a cytochrome P450-dependent pathway
Categories:
Transfection 
Authors:
Gehlhaus M, Schmitt N, Volk B, Meyer R 
In:
J Pharmacol Exp Ther (2007) 322(2): 550-9 
The Nogo-66 receptor NgR1 is required only for the acute growth cone-collapsing but not the chronic growth-inhibitory actions of myelin inhibitors
Categories:
Transfection 
Authors:
Chivatakarn O, Kaneko S, He Z, Tessier-Lavigne M, Giger RJ 
In:
J Neurosci (2007) 27(27): 7117-24 
Sumoylation in axons triggers retrograde transport of the RNA-binding protein La
Categories:
Transfection 
Authors:
van Niekerk EA, Willis DE, Chang JH, Reumann K, Heise T, Twiss JL 
In:
Proc Natl Acad Sci USA (2007) 104(31): 12913-8 
Neogenin-RGMa Signaling at the Growth Cone Is Bone Morphogenetic Protein-independent and Involves RhoA, ROCK, and PKC
Categories:
Transfection 
Authors:
Conrad S, Genth H, Hofmann F, Just I, Skutella T 
In:
J Biol Chem (2007) 282(22): 16423-33 
KLHL1/MRP2 mediates neurite outgrowth in a GSK3-dependent manner
Categories:
Transfection 
Authors:
Seng S, Avraham HK, Jiang S, Venkatesh S, Avraham S 
In:
Mol Cell Biol (2006) 26(22): 8371-84 
Regulation of the Raf-MEK-ERK pathway by protein phosphatase 5
Categories:
Transfection 
Authors:
von Kriegsheim A, Pitt A, Grindlay GJ, Kolch W, Dhillon AS 
In:
Nat Cell Biol (2006) 8(9): 1011-6 
Intersectin-1L nucleotide exchange factor regulates secretory granule exocytosis by activating Cdc42
Categories:
Transfection 
Authors:
Malacombe M, Ceridono M, Calco V, Chasserot-Golaz S, McPherson PS, Bader MF, Gasman S 
In:
EMBO J (2006) 25(15): 3494-503 
CIB1, a ubiquitously-expressed Ca2+-binding protein-ligand of the InsP3 receptor Ca2+-release channel
Categories:
Transfection 
Authors:
White C, Yang J, Monteiro MJ, Foskett JK 
In:
J Biol Chem (2006) 281(30): 20825-20833 
Regulation of Neuroendocrine Exocytosis by the ARF6 GTPase-activating Protein GIT1
Categories:
Transfection 
Authors:
Meyer MZ, Deliot N, Chasserot-Golaz S, Premont RT, Bader MF, Vitale N 
In:
J Biol Chem (2006) 281(12): 7919-26 
Rac1 Leads to Phosphorylation-dependent Increase in Stability of the p66shc Adaptor Protein: Role in Rac1-induced Oxidative Stress
Categories:
Transfection 
Authors:
Khanday FA, Yamamori T, Mattagajasingh I, Zhang Z, Bugayenko A, Naqvi A, Santhanam L, Nabi N, Kasuno K, Day BW and Irani K 
In:
Mol Biol Cell (2006) 17(1): 122-129 
A prohormone convertase cleavage site within a predicted alpha-helix mediates sorting of the neuronal and endocrine polypeptide VGF into the regulated secretory pathway
Categories:
Transfection 
Authors:
Garcia AL, Han SK, Janssen WG, Khaing ZZ, Ito T, Glucksman MJ, Benson DL and Salton SR 
In:
J Biol Chem (2005) 280(50): 41595-41608 
The intracellular domain of p75NTR as a determinant of cellular reducing potential and response to oxidant stress
Categories:
Transfection 
Authors:
Tyurina YY, Nylander KD, Mirnics ZK, Portugal C, Yan C, Zaccaro C, Saragovi HU, Kagan VE and Schor NF 
In:
Aging Cell (2005) 4(4): 187-196 
Involvement of a Rac activator,P-Rex1, in neurotrophin-derived signaling and neuronal migration
Categories:
Transfection 
Authors:
Yoshizawa M, Kawauchi T, Sone M, Nishimura YV, Terao M, Chihama K, Nabeshima Y and Hoshino M 
In:
J Neurosci (2005) 25(17): 4406-4419 
Steroid receptor coactivator-1 splice variants differentially affect corticosteroid receptor signaling
Categories:
Transfection 
Authors:
Meijer OC, Kalkhoven E, van der Laan S, Steenbergen PJ, Houtman SH, Dijkmans TF, Pearce D and de Kloet ER 
In:
Endocrinology (2005) 146(3): 1438-1448 
Defining the CREB Regulon; A Genome-Wide Analysis of Transcription Factor Regulatory Regions
Categories:
Transfection 
Authors:
Impey S, McCorkle SR, Cha-Molstad H, Dwyer JM, Yochum GS, Boss JM, McWeeney S, Dunn JJ, Mandel G and Goodman RH 
In:
Cell (2004) 119(7): 1041-1054 
A molecular basis underlying differences in the toxicity of botulinum serotypes A and E
Categories:
Transfection 
Authors:
Bajohrs M, Rickman C, Binz T and Davletov B 
In:
EMBO Rep (2004) 5(11): 1090-1095 
New non-viral method for gene transfer into primary cells
Categories:
Transfection 
Authors:
Gresch O, Engel FB, Nesic D, Tran TT, England HM, Hickman ES, Korner I, Gan L, Chen S, Castro-Obregon S, Hammermann R, Wolf J, Muller-Hartmann H, Nix M, Siebenkotten G, Kraus G and Lun K 
In:
Methods (2004) 33(2): 151-163 
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