Met-1fvb2

mammary epithelial carcinoma. Derived from a FVB/N-Tg (MMTV-PyVmT) tumor exhibits invasive growth and 100% metastases when transplanted into the females FVB/N mammary fat pad. Clinical and Exp. Metastasis, Volume 22, Number 1,2005 , pp.47-59.
Cell Type:
Mammary Epith.
Tissue Origin:
breast
Species:
mouse
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent

Recommended Media

DMEM:F12 combines the richness of F12 with the higher component concentrations of DMEM. This medium is well suited for clonal density cultures and has been extensively used to demonstrate the effect of various hormones and growth factors on target tissues.

Storage = 2ºC to 8ºC

12-719 = 3.151 g/L glucose, L-glutamine and 15mM HEPES
BE04-687Q = 3.151 g/L glucose and L-glutamine, without HEPES
BE04-687F/U1 = 3.151 g/L glucose and Ultraglutamine I, without HEPES

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
V T-030 1e6 95% 90% Plasmid (general) 2 µg 100 µl I/II/2b