Plasmodium falciparum

P. falciparum is a protozoan parasite, one of the species of Plasmodium that cause malaria in humans.

Cell Type:
Plasmodium
Tissue Origin:
Species:
unicellular
Research Area:
Parasitology
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
P3

CM-150

90%


5 µg 20 µl Shuttle
P3 CM-138 90% 5 µg 20 µl Shuttle

Citations (10)

Categories:
Transfection, Protozoa 
Authors:
Wilde ML1,2, Triglia T1, Marapana D1, Thompson JK1, Kouzmitchev AA3, Bullen HE4, Gilson PR4,5, Cowman AF1,2, Tonkin CJ6,2. 
In:
mBio (2019) 10: 5 
Categories:
Protozoa 
Authors:
López-Gutiérrez B1, Cova M1, Izquierdo L1. 
In:
Parassitologia (2019) :  
Categories:
Transfection 
Authors:
Low LM#1, Azasi Y#1, Sherling ES#1,2, Garten M3, Zimmerberg J3, Tsuboi T4, Brzostowski J5, Mu J1, Blackman MJ2,6, Miller LH7. 
In:
mBio (2019) 10: 4 
Categories:
Transfection 
Authors:
Grzybek M1, Golonko A2, Górska A3, Szczepaniak K3, Strachecka A4, Lass A5, Lisowski P6,7. 
In:
Biotechnology Journal (2018) 102: 11 
Categories:
Transfection 
Authors:
Crawford ED1,2,3, Quan J1,2,3, Horst JA2, Ebert D2,3, Wu W2, DeRisi JL1,2,3. 
In:
PLoS ONE (2017) 12: 5 
Categories:
Transfection 
Authors:
Hill RJ, Ringel A, Knuepfer E, Moon RW, Blackman MJ, van Ooij C. 
In:
J Biol Chem (2016) 10(1): 1-30 
Categories:
Transfection 
Authors:
Molina-Cruz A1, Canepa GE2, Kamath N2, Pavlovic NV2, Mu J2, Ramphul UN2, Ramirez JL2, Barillas-Mury C1. 
In:
Proc Natl Acad Sci USA (2015) 49: 15178(83) 
Categories:
Primary Cells and Media 
Authors:
Dumoulin PC1, Trop SA1, Ma J1, Zhang H1, Sherman MA2, Levitskaya J1 
In:
PLoS ONE (2015) 10(6): 0129623 
Categories:
Transfection 
Authors:
Mehdi Ghorbal, Molly Gorman, Cameron Ross Macpherson, Rafael Miyazawa Martins, Artur Scherf, Jose-Juan Lopez-Rubio 
In:
Nat Biotechnol (2014) 32(8): 819-21 
Categories:
Transfection 
Authors:
Collins CR1, Das S, Wong EH, Andenmatten N, Stallmach R, Hackett F, Herman JP, Müller S, Meissner M, Blackman MJ. 
In:
Mol Microbiol (2013) 88(4): 687-701 
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