cells

P. falciparum is a protozoan parasite, one of the species of Plasmodium that cause malaria in humans.

Cell Type:

Plasmodium

Tissue Origin:

Species:

unicellular

Research Area:

Parasitology

Cell Characteristics:

Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Protocol	Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
	P3	CM-150		90%			5 µg	20 µl	96-well
	P3	CM-138		90%			5 µg	20 µl	96-well

Citations (10)

Protein Kinase A Is Essential for Invasion of Plasmodium falciparum into Human Erythrocytes.

Categories:

Transfection, Protozoa

Authors:

Wilde ML1,2, Triglia T1, Marapana D1, Thompson JK1, Kouzmitchev AA3, Bullen HE4, Gilson PR4,5, Cowman AF1,2, Tonkin CJ6,2.

In:

mBio (2019) 10: 5

A Plasmodium falciparum C-mannosyltransferase is dispensable for parasite asexual blood stage development.

Categories:

Protozoa

Authors:

López-Gutiérrez B1, Cova M1, Izquierdo L1.

In:

Parassitologia (2019) :

Deletion of Plasmodium falciparum Protein RON3 Affects the Functional Translocation of Exported Proteins and Glucose Uptake.

Categories:

Transfection

Authors:

Low LM#1, Azasi Y#1, Sherling ES#1,2, Garten M3, Zimmerberg J3, Tsuboi T4, Brzostowski J5, Mu J1, Blackman MJ2,6, Miller LH7.

In:

mBio (2019) 10: 4

The CRISPR/Cas9 system sheds new lights on the biology of protozoan parasites.

Categories:

Transfection

Authors:

Grzybek M1, Golonko A2, Górska A3, Szczepaniak K3, Strachecka A4, Lass A5, Lisowski P6,7.

In:

Biotechnology Journal (2018) 102: 11

Plasmid-free CRISPR/Cas9 genome editing in Plasmodium falciparum confirms mutations conferring resistance to the dihydroisoquinolone clinical candidate SJ733

Categories:

Transfection

Authors:

Crawford ED1,2,3, Quan J1,2,3, Horst JA2, Ebert D2,3, Wu W2, DeRisi JL1,2,3.

In:

PLoS ONE (2017) 12: 5

Regulation and essentiality of the START-domain-containing phospholipid transfer protein PFA0210c in malaria parasites.

Categories:

Transfection

Authors:

Hill RJ, Ringel A, Knuepfer E, Moon RW, Blackman MJ, van Ooij C.

In:

J Biol Chem (2016) 10(1): 1-30

Plasmodium evasion of mosquito immunity and global malaria transmission: The lock-and-key theory.

Categories:

Transfection

Authors:

Molina-Cruz A1, Canepa GE2, Kamath N2, Pavlovic NV2, Mu J2, Ramphul UN2, Ramirez JL2, Barillas-Mury C1.

In:

Proc Natl Acad Sci USA (2015) 49: 15178(83)

Flow Cytometry Based Detection and Isolation of Plasmodium falciparum Liver Stages In Vitro

Categories:

Primary Cells and Media

Authors:

Dumoulin PC, Trop SA, Ma J, Zhang H, Sherman MA, Levitskaya J

In:

PLoS ONE (2015) 10(6): 0129623

Genome editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9 system

Categories:

Transfection

Authors:

Mehdi Ghorbal, Molly Gorman, Cameron Ross Macpherson, Rafael Miyazawa Martins, Artur Scherf, Jose-Juan Lopez-Rubio

In:

Nat Biotechnol (2014) 32(8): 819-21

Robust inducible Cre recombinase activity in the human malaria parasite Plasmodium falciparum enables efficient gene deletion within a single asexual erythrocytic growth cycle.

Categories:

Transfection

Authors:

Collins CR1, Das S, Wong EH, Andenmatten N, Stallmach R, Hackett F, Herman JP, Müller S, Meissner M, Blackman MJ.

In:

Mol Microbiol (2013) 88(4): 687-701

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