FRT

FRT (Fisher rat thyroid). Derived from normal thyroids of Fischer rats.
Cell Type:
Thyroid Epith.
Tissue Origin:
thyroid
Species:
rat
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
L L-029 88% 96% Plasmid (general) 2 µg 100 µl I/II/2b
L X-001 89% 97% Plasmid (general) 2 µg 100 µl I/II/2b
V L-029 77% 85% Plasmid (general) 2 µg 100 µl I/II/2b
V X-001 87% 96% Plasmid (general) 2 µg 100 µl I/II/2b

Citations

Categories:
 
Authors:
Valentine CD, Zhang H, Phuan PW, Nguyen J, Verkman AS, Haggie PM. 
In:
Cell Microbiol (2014) 16(1): 1-14