cells

Cloned from pupal ovarian tissue. The SF-21 cell line is highly susceptible to infection with Autographa california nuclear polyhedrosis virus (AcNPV baculovirus) and can be used with all baculovirus expression vectors;high expression levels are observed.

Cell Type:

Insect

Tissue Origin:

Species:

spodoptera frugiperda (army fall worm)

Cell Characteristics:

Suspension

Recommended Media

Insect-XPRESS

Insect-XPRESS™ Protein-free Insect Cell Medium is a formulation designed to support the growth of insect cell lines derived from Spodoptera frugiperda (Sf9 and Sf21).
Cell densities in excess of 8.3 x 10^6 cells/ml can be achieved with suspension cultures of Sf9 cells using Insect-XPRESS™ Medium and an excess of oxygen. This formulation can also be used for stationary mono layer cultures and shake-flask cultures.
Insect-XPRESS™ Medium contains L-glutamine and supports superior production of recombinant proteins when using the Baculovirus Expression Vector System (BEVS).

Storage = 2°C to 8°C

Transfection Information

In case no data are shown for the selected cell type, please take a look at our optimization strategy or contact our Scientific Support Teams to get further guidance on how to easily determine optimal Nucleofection conditions yourself.