Cloned from pupal ovarian tissue. The SF-21 cell line is highly susceptible to infection with Autographa california nuclear polyhedrosis virus (AcNPV baculovirus) and can be used with all baculovirus expression vectors;high expression levels are observed.

Cell Type:
Tissue Origin:
spodoptera frugiperda (army fall worm)
Cell Characteristics:

Recommended Media

A modification of Grace's Insect Medium developed by Drs. Gardiner and Stockdale, this medium optimizes the expression of baculovirus grown in cultured cells derived from the fall army worm, Spodoptera frugiperda. It is termed BML-TC/10, but is more commonly referred to as TC-100.

Storage = 2ºC to 8ºC 

Grace's Insect Medium was developed in 1962 and has been shown, with the addition of serum, to support the growth of insect cells, including Spodoptera frugiperda. Unsupplemented Grace's Insect Culture Medium is ideal for transfection because it does not contain lactalbumin hydrolysate, or yeastolate which can interfere with transfection.
When supplemented with L-glutamine, lactalbumin hydrolysate, and yeastolate Grace's Insect Medium can be used for general growth and maintenance of insect cells.

Storage = 2ºC to 8ºC

04-457 has no lactalbumin hydrolysate, yeastolate, gentamycin or FBS
04-649 has lactalbumin hydrolysate, yeastolate, and gentamycin, but no FBS 
04-501 Grace's Complete Insect Medium - has lactalbumin hydrolysate, yeastolate, gentamycin and 10% heat-inactivated FBS 

Insect-XPRESS™ Protein-free Insect Cell Medium is a formulation designed to support the growth of insect cell lines derived from Spodoptera frugiperda (Sf9 and Sf21).
Cell densities in excess of 8.3 x 10^6 cells/ml can be achieved with suspension cultures of Sf9 cells using Insect-XPRESS™ Medium and an excess of oxygen. This formulation can also be used for stationary mono layer cultures and shake-flask cultures.
Insect-XPRESS™ Medium contains L-glutamine and supports superior production of recombinant proteins when using the Baculovirus Expression Vector System (BEVS).

Storage = 2°C to 8°C

Transfection Information

In case no data are shown for the selected cell type, please take a look at our optimization strategy or contact our Scientific Support Teams to get further guidance on how to easily determine optimal Nucleofection conditions yourself.