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(BTI-TN-5B1-4) are a clonal isolate, derived from the parental Trichopulsia ni (Cabbage Looper) cell line and are commonly used for the expression of recombinant proteins

Cell Type:
Insect
Tissue Origin:
Species:
Trichopulsia ni (Cabbage Looper)
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SF CD-141 4e5 94% ±5 73% ±7 Plasmid (general) 1 µg 20 µl 4D X-Unit
SF CD-141 2e6 93% ±4 77% ±15 Plasmid (general) 5 µg 100 µl 4D X-Unit

Citations

Tuning cytokine receptor signaling by re-orienting dimer geometry with surrogate ligands
Categories:
Serum-free and Speciality Media 
Authors:
Moraga I, Wernig G, Wilmes S, Gryshkova V, Richter CP, Hong WJ, Sinha R, Guo F, Fabionar H, Wehrman TS, Krutzik P, Demharter S, Plo I, Weissman IL, Minary P, Majeti R, Constantinescu SN, Piehler J, Garcia KC. 
In:
Cell (2015) 160(6): 1196-208 
Specific activation of the TLR1-TLR2 heterodimer by small-molecule agonists
Categories:
Serum-free and Speciality Media 
Authors:
Cheng K, Gao M, Godfroy JI, Brown PN, Kastelowitz N, Yin H 
In:
Sci Adv. (2015) 01(3): e1400139 
Calsyntenin-3 molecular architecture and interaction with neurexin 1a.
Categories:
Serum-free and Speciality Media 
Authors:
Lu Z, Wang Y, Chen F, Tong H, Reddy MV, Luo L, Seshadrinathan S, Zhang L, Holthauzen LM, Craig AM, Ren G, Rudenko G. 
In:
J Biol Chem (2014) 289(50): 34530-42 
Enhanced expression of full-length human cytomegalovirus fusion protein in non-swelling baculovirus-infected cells with a minimal fed-batch strategy
Categories:
Serum-free and Speciality Media 
Authors:
Patrone M, Carinhas N, Sousa MQ, Peixoto C, Ciferri C, Carfì A, Alves PM 
In:
PLoS ONE (2014) 9(3): e90753 

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