BV2

microglial cells

Cell Type:
Glial Cells
Tissue Origin:
brain
Species:
mouse
Research Area:
Neurobiology
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SF CM-158 2e5 65-70% 70-75% Plasmid (general) 0.4 µg 20 µl Shuttle
SF CM-158 2e5 60-70% 68% Plasmid (general) 400 ng 20 µl 4D X-Unit

Citations

Categories:
Transfection 
Authors:
Sui Z, Sniderhan LF, Schifitto G, Phipps RP, Gelbard HA, Dewhurst S, Maggirwar SB 
In:
J Immunol (2007) 178(5): 3226-36