Dendritic cell, mouse, mature - C57BL/6
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Mature dendritic cells (antigen-presenting cells (APC) that activate T lymphocytes)
Cell Type:
Dendritic cell (DC)
Tissue Origin:
blood
Species:
mouse
Research Area:
Immunotherapy / Hematology
Cell Characteristics:
Suspension
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
P3
DN-107
3e4
24-34%
86-90%
Plasmid (general)
0.4 µg
20 µl
4D X-Unit
P3
96-DN-107
3e4
29% ±5
88% ±2
Plasmid (general)
0.4 µg
20 µl
96-well
DC, mouse
AN-001
3e5
37% ±3
63% ±5
Plasmid (general)
2 µg
100 µl
I/II/2b
Citations
Impaired Bcl3 up-regulation leads to enhanced LPS-induced IL-23p19 gene expression in IL-10-/- mice
Categories:
Transfection
Authors:
Muehlbauer M, Chilton PM, Mitchell TC, Jobin C
In:
J Biol Chem (2008) 283(21): 14182-9